1、 General White paper The RNGS stability test.A refined method for assessing shelf life stability of RNGS beverages General Publisher Tetra Pak Processing Systems AB SE 221 86 Lund,Sweden Published October 2015 Tetra Pak International S.A No part of this publication may be duplicated in any form with

2、out the source being indicated.3 CONTENTS Summary_ 4 Advantages of an accelerated method _ 5 The RNGS stability test in brief _ 7 Particle sedimentation in peanut milk _ 7 Soy milk creaming _ 9 Discussion/conclusions _ 11 What the method does not assess _ 12 Tetra Pak your processing partner _ 13 4

3、SUMMARY Beverages based on rice,nuts,grains and/or soybeans(RNGS)are among the fastest growing in many markets today and forecasts indicate that growth will be sustained or even increase.This is an exciting product group that involves almost endless possibilities for new formulations and combination

4、s.The RNGS raw materials represent a wide spectrum of characteristics.It is not only hundreds of different ingredients and thousands of different combinations of raw materials that are used.One must also take into account the variations depending on the season,growing conditions,where the crop is gr

5、own etc.All in all,this means new challenges for the lab,for example when it comes to assessing shelf life stability of these products.A development of the NIZO-method for measuring homogenization efficiency in dairy milk has shown promising results for the assessment of two important parameters for

6、 shelf life stability in RNGS beverages,namely creaming and sedimentation.Properly used,calibrated and correlated to real time storage tests,this method the RNGS stability test has the potential to serve as an important aid in product development by giving crucial input to the assessment of a produc

7、ts life cycle.Also,this quick,simple and effective method for measuring homogenization efficiency can contribute to minimizing the need for additives such as stabilizers and emulsifiers,at the same time reducing energy costs.This white paper provides an introduction to the development of an objectiv

8、e method for measuring shelf life stability of RNGS products the RNGS stability test.The aim is to spread the method so that it can be introduced and evaluated in many different contexts and for different products.5 ADVANTAGES OF AN ACCELERATED METHOD The only completely reliable way to assess shelf

9、 life stability of a product is to simply put it on the shelf and wait.Analyzing the product periodically will provide an answer whether a particular product survived one,two,three,or maybe six months on the shelf without unacceptable changes.It goes without saying that this very time-consuming meth

10、od means limitations in,for example,product development,where it would be of tremendous help being able to quickly and safely obtain reliable data on how a product or a slightly modified formulation behaves in storage.The same applies in the continuous quality control,where seasonal variations or ch

11、ange of supplier may mean that the process must be adjusted for the product to maintain consistent and high quality over time.An important parameter for determining a products shelf life stability is to measure homogenizing efficiency.Today there is a variety of methods for making such measurements.

12、The most common is the so called NIZO method,which involves centrifuging a sample of milk for 30 minutes and then dividing the fat content of the bottom layer with the fat content of the whole sample.By multiplying the quota by 100,an index is obtained.The higher the index,the more efficient the hom

13、ogenization.A typical NIZO index of pasteurized milk is 50-80.Unfortunately,this method does not provide the necessary information about RNGS products.For the consumer,RNGS products usually appear as simple and natural.Rice,nuts,grains and/or soybeans that are processed just enough to retain their t

14、aste,their nutritional content and other good qualities.These are also important selling points behind the products rising popularity.But if you look at these products from a food processors perspective,they are often anything but simple.It places great demands to scale up for example a peanut or ca

15、shew nut milk from small scale preparation to an industrially processed,attractive product with high and consistent quality,ready for the mass market.Formulations with input of multiple raw materials,with completely different characteristics,also mean both new opportunities on the market and challen

16、ges for the production processes.Two typical characteristics of RNGS-products are that they contain particles that will cause sedimentation and that the fat that they contain tends to give rise to so-called creaming.Sedimentation is a minor problem,as”shaking the container”is today a learned and acc

17、epted consumer behaviour-just as one shakes a pack of,for example,orange juice containing pulp and fibre.Provided that the sediment hasnt formed a hard bottom layer,the shaking will restore the emulsion and the product will be perceived as smooth and fine.Creaming,on the other hand,is a bigger probl

18、em,partly because the fat bubbles that may form do not dissolve as easily and,in addition,are perceived as unappetizing both in appearance,taste and mouthfeel.The more stable a product is over its shelf life cycle,the less problems will be caused by sedimentation and creaming,and an important key is

19、 to strike the right homogenization efficiency.6 With the right homogenizing efficiency,both sedimentation and creaming can be maintained at controlled,foreseeable and acceptable levels.The product can be given the right quality with respect to the sensory characteristics-taste,mouthfeel,viscosity,e

20、tc.-without unnecessary additives such as stabilizers and emulsifiers.Also,”over-homogenization”can be avoided,meaning both higher product quality and reduced energy costs.But the characteristics and properties of a product at the moment it leaves the production line is one thing.How it tastes and i

21、s perceived when it finally reaches the consumer after a shorter or longer period on the store shelf can unfortunately be something completely different.For product developers and quality managers,it is therefore of great importance to be able to predict how a product is maintained over time.To simp

22、ly place a product on the shelf and wait is an option,but not an effective one.The product developer wants to be able to experiment with different formulations and quickly get an answer,and there are also other obvious reasons for being able to assess the products shelf life stability before placing

23、 it on the market.A further development of test methods used to measure homogenization efficiency has shown to give results that correlate with real-time storage and could be a major step on the road to obtain an objective measurement of shelf life stability of RNGS products.7 THE RNGS STABILITY TES

24、T IN BRIEF The method,developed in collaboration with two world leading producers of RNGS products,aims to objectively predict the shelf life stability without a need of real life storage tests.The problem to solve has been whether it is possible to achieve correlation between a fast and effective m

25、ethod an accelerated test of various products and values and the same products stored under various periods of time.The test method has been developed for measuring two different parameters,namely,particle sedimentation and creaming.Here we describe two typical results of accelerated and real-time t

26、ests of peanut milk and soymilk.Where ni is the number-based frequency of particles in size class i having a diameter of D Particle sedimentation in peanut milk A fresh sample was centrifuged for 15 minutes with a G-force from 10 to 1.000g.The sample was then divided into three parts-top,middle and

27、bottom and particle size analysis D5,3 was performed on the top layer.For correlation,the results were compared with particle size analysis results of the same products after different times of storage.The theory behind using only the top layer to assess sedimentation instead of the bottom layer,whe

28、re the most of the sedimentation collected is that the top layer is changing most rapidly,both during centrifugation and storage.By analyzing the presence of particles after centrifugation at different speeds and lengths of time,one can determine how sedimentation will occur in the product.D5;3=(niD

29、i5)/(niDi3)8 Figure 1.The centrifuged sample is divided into three layers-the sample is taken from the top layer.Comparison between real and accelerated results The graph shows the correlation between real-time and accelerated shelf life tests.Centrifugation of peanut milk for 15 minutes at 500g can

30、 mimic its sedimentation after 2 months.9 Soy milk creaming Fresh samples of soymilk were centrifuged to 1.000 to 2.000g for 15 and 30 minutes respectively.The samples were then divided into five layers.Particle size analysis was performed on the bottom layer.By analogy with the theory behind the pa

31、rticle sedimentation analysis,the theory behind this is that the fat globules leave the bottom layer most quickly,so by examining this layer the degree and speed of the product creaming can be examined.These tests have been correlated with samples of products stored for different lengths of time.Fig

32、ure 2.The centrifuged sample is divided into five layers-the sample is taken from the bottom layer.10 Accelerated test of soy milk With more intense centrifugation,the particle size of the bottom layer becomes smaller-the curve moves to the left.Real shelf life performance of soy milk After 4 weeks

33、of storage,D5,3 is between the values of the two accelerated tests(1.000g/15 min and 1.000g/30 min).Table 1.Comparison between the real and the accelerated results.Samle Name D5,3 Vita HK premium 0 day 0.474 Vita HK premium 1000-15min-5 0.472 Vita HK premium 1000-30min-5 0.445 Vita HK premium 2000-3

34、0min-5 0.438 Vita HK premium 4 weeks-5 0.462 11 DISCUSSION/CONCLUSIONS The attempts made to obtain a quick,simple and safe method for the objective assessment of shelf life stability of RNGS products have so far yielded very promising results.With proper calibration and further testing,there are str

35、ong indications that centrifugation and sample analysis according to the method described above will be useful to reduce the need for time-consuming real-time shelf life stability tests considerably.Today there are correlated values for a number of products and this number is growing constantly.But

36、for the method to become truly useful and accepted,further testing and development work is required,with the task to establish exact correlations between the accelerated test results and storage tests.A desirable scenario would be that the method was spread,used and evaluated by producers of RNGS be

37、verages around the globe.In this way,the method can be refined while an increasingly large database containing reliable shelf life stability numbers products with different ingredients and formulations can be built up.12 WHAT THE METHOD DOES NOT ASSESS The RNGS stability test is intended to assess s

38、helf life stability in RNGS products with respect to the two important parameters of sedimentation and creaming by measuring homogenization efficiency.Other parameters,such as biological stability,protein stability,coagulation of fat etc.must be measured by established methods.In some cases,such as

39、when the product contains raw material from both RNGS and dairy milk,it is difficult to find a significant change in particle size distribution,but at the same time,in those products there is a usually huge change in the fat content.In those cases,fat content analysis,using a laser diffraction instr

40、ument,is a more suitable method for assessing shelf life stability.Particle size distribution in peanut milk comparison between accelerated and real time tests The difference in results between accelerated test and storage test is caused by fat coagulation.13 TETRA PAK YOUR PROCESSING PARTNER Tetra

41、Paks development engineers,process engineers,designers and field service engineers work with you to create solutions for obtaining optimal quality and shelf life stability in beverages based on rice,nuts,grains and soybeans.At our Product Development Centers you can experiment with recipes and use t

42、he latest processing equipment for RNGS beverages.You will have the full support of our food technologists and scientists.When applicable,we will use the newly developed RNGS stability test,as described above,to provide crucial information to your development process.In addition to saving time and m

43、oney by being able to quickly assess the shelf life of your product,this method can be very helpful to reach a consistent high quality and making the most out of the raw materials used in your product.Typical results could include a reduced energy cost and a minimized need of additives such as stabilizers and emulsifiers.Feel free to contact your Tetra Pak representative or connect with us via our http:/ Tetra Pak and Protects Whats Good are trademarks belonging to the Tetra Pak G Tetra Pak International S.A,Tetra Pak Processing system AB,10-2015