李建麗-流行病學調查：動物疫病防控的原點.pdf

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1、流行病學調查：動物疫病防控的原點！Epidemiological investigation:the starting point of animal disease prevention and control!李建麗 Li Jianli 河南農業大學Henan Agricultural University2024年04月26日April 26,2024一帶一路國際生豬產業大會提 要 Abstractp 流行病學的核心要點Key Points of Epidemiologyp 常見豬病的流行現狀Epidemiological status of common swine diseasesp

2、 流行病學的臨床價值Clinical value of epidemiologyp 流行病學的未來挑戰Future challenges of epidemiology一帶一路國際生豬產業大會一一流行病學的核心要點Key Points of Epidemiology一帶一路國際生豬產業大會形成一個統一Form a unity豬Pig病Disease人Human產品PRODUCT路線Route生態Ecology一帶一路國際生豬產業大會兩個關鍵指標Two key indicators流行率Prevalence發病率Morbidity流行病學調查Epidemiological investigati

3、on一帶一路國際生豬產業大會采用兩種技術Using two technologies技技術術手手段段Technical means分分子子病病原原學學Molecular etiology血血清清學學SerologyP PC CR R/R RT T-P PC CR R熒熒光光定定量量P PC CR RFluorescence quantitative PCR熒熒光光P PC CR RFluorescence PCR分分子子病病原原學學Molecular etiologyL LA AM MP P微微流流孔孔P PC CR RMicroflow well PCR數數字字P PC CR RDigita

4、l PCR基基因因測測序序與與分分析析Gene sequencing and analysis宏宏基基因因組組學學MetagenomicsE EL LI IS SA AH HI I化化學學發發光光Chemiluminescence試試紙紙條條Test strips一帶一路國際生豬產業大會完成三個任務Complete three tasks尋根Trace溯源Investigate防控Prevention and control查明傳染源Identify the source of infection找出傳播途徑Find out the route of transmission保護易感動物Pro

5、tection of susceptible animals一帶一路國際生豬產業大會劃分四個層次Divided into four levels保護Protection 健康 Healthy疫病Epidemic disease危害Hazards流行Epidemic病學Disease元兇是誰Who is the culprit?發病率Incidence死亡率Mortality rate個體治療Individual therapy群體免疫Herd immunity豬場整群Pig farm herd區域聯防Regional joint Prevention國家聯控National Joint Con

6、trol生物安全Biosafety流行率Prevalence示病癥狀Disease-indicating symptoms病理變化Pathological changes一帶一路國際生豬產業大會是什么？What is it?來自哪里？Where does it come from?要去哪里？Where are they going?危害多大？How harmful is it?如何防控？How to prevent and control?回答五個問題Answer the five questionsFigure 2.Global genotype distribution of porcin

7、e RVA strains reported in historic(1976-2011,bluefigure arrows)and current(after 2000,pink figure arrows)studies.Porcine RVAs are also detected in Germany and Russia,but no genotyping data is available.圖 2.歷史研究（1976-2011 年，藍色圖箭頭）和當前研究（2000 年后，粉色圖箭頭）中報告的豬 RVA 株系的全球基因型分布。德國和俄羅斯也檢測到豬 RVA，但沒有基因分型數據。Porc

8、ine Rotaviruses:Epidemiology,Immune Responses and Control Strategies豬豬輪輪狀狀病病毒毒：流流行行病病學學、免免疫疫反反應應和和控控制制策策略略一帶一路國際生豬產業大會群體性Collectivity對比性Comparability概率性Probability溯源性Traceability預防性Prophylactic動態性Dynamic關注六個特點Focus on six characteristics劉娜 國際病毒學雜志 2022 年 4 月第 29 卷第 2 期 Liu Na International Journal o

9、f Virology,Volume 29,Issue 2,April 2022 一帶一路國際生豬產業大會二二II常見豬病的流行現狀Epidemiological status of common swine diseases一帶一路國際生豬產業大會p 整體：北非南瀉，藍耳多樣 Overall:ASF in the North,Diarrhea in the South,PRRS Diverse 藍耳：豬場頑疾，NADC30占優 PRRS:Chronic disease in pig farm,NADC30 is dominant 非瘟：嚴重頭疼，弱毒重組占優 ASF:Severe headac

10、he,attenuated/recombinant is superior 腹瀉：主流PEDV，PoRV上升態勢 Diarrhea:Mainstream PEDV,PoRV on the rise 圓環：PCV2d占優，PCV3抬頭 PCV:PCV2d predominates,PCV3 heads up 偽狂：基因變異，免疫有效 PR:Genetic variation,effective immunization 豬瘟：免疫穩定，偶有點發 Classical swine fever:Stable immunity,occasional spotting 口蹄疫：總體平穩，O型為主 Foot

11、-and-mouth disease:Overall stable,mainly type O 細菌：鏈球副豬仍是主流，警惕APP、APP和 LI Bacteria:SS and HPS is still the mainstream,Beware of APP,APP and LI 一帶一路國際生豬產業大會1、PRRS：豬場頑疾，NADC30-like占優勢！1.PRRS:For chronic diseases in pig farms,NADC30-like has the advantage!199019912001*c PRRSV歐美流行期European and American

12、epidemic period 2006高熱病High fever2010 2012*2013NADC30-like c PRRSV中國流行期Epidemic period in China c PRRSV 變異期 Period of variation h PRRSV 爆發期 Period of outbreak多毒株并存 Coexistence of multiple strains 免疫抑制期Period of immunosuppressionNADC34-like201720201987美國United States首次First time德國Germany首次First time荷

13、蘭Netherlands歐洲型European type1995中國首次First time in China1992美國United States美洲型American type高致病報道Highly pathogenic report高致病性疫苗強制免疫Compulsory immunization with highly pathogenic vaccine 2000 2004PCV2aPCV2bPCV3PCV2d美國出現The United States appeared1-4-4毒株1-4-4 strain 流行歷史 History of prevalence一帶一路國際生豬產業大會

14、 遺傳分支多樣 Variety of genetic branches一帶一路國際生豬產業大會 多毒株并存：類NADC30（L1.8）為主要流行毒株，同時存在HP-PRRS、重組毒株、弱毒疫苗衍生毒株等。Coexistence of multiple strains:NADC30-like(L1.8)is the main prevalent strain,and there are also HP-PRRS,recombinant strains,attenuated vaccine-derived strains,etc.遺傳多樣性更復雜：NSP2缺失模式多樣性、進化分支多樣性。Genet

15、ic diversity is more complex:diversity in NSP2 deletion patterns and diversity in evolutionary branches.新的PRRS分支不斷出現：類NADC34（L1.5）、QYYZGM2（L3）、PRRS1-4-4。New PRRS branches continue to emerge:Class NADC34(L1.5）、QYYZGM2（L3),PRRS1-4-4.歐洲型毒株：局部地區正悄然流行。European strain:It is quietly endemic in some areas.多

16、種疫苗弱毒株并存：弱毒返強、衍生、重組的風險加大。Coexistence of attenuated strains in multiple vaccines:the risk of reversion to virulence,derivation and recombination of attenuated viruses is increased.臨床發病表現差異大：持續性感染、免疫抑制、陽性不穩定豬場、陽性穩定豬場、陰性豬場同在。The clinical manifestations are quite different:persistent infection,immunosup

17、pression,unstable positive pig farms,stable positive pig farms and negative pig farms are the same.流行態勢：多毒株并存 Epidemic situation:coexistence of multiple strains一帶一路國際生豬產業大會2、腹瀉類病原：PEDV 仍是主流，PoRV 上升態勢2.Diarrhea pathogens:PEDV is still the mainstream,PoRV is on the rise一帶一路國際生豬產業大會G2基因群G2 gene groupG1

18、基因群G1 gene groupG2b類INDEL毒株INDEL-like strainG1aG1bG2a（1）PEDV：G2a分支占優，警惕重組毒株！(1)PEDV:The G2a branch is dominant,beware of recombinant strains!依據S基因對2020-2022年分離毒株進化分析：Evolutionary analysis of strains isolated from 2020 to 2022 based on S gene:G2a分支：為主流，占70-80%G2a branch:Mainstream,accounting for 70-8

19、0%G2b分支：多為現有疫苗株G2b branch:mostly existing vaccine strainsS-INDEL毒株：S-INDEL strain:G1a：經典毒株或疫苗毒株G1a:Classical or vaccine strainG1b：重組毒株G1b:Recombinant strains提示：PEDV流行毒株基因型呈多樣性，以G2a分支為主流，同時警惕和監控重組毒株。hint:The genotypes of PEDV epidemic strains are diverse,with the G2a branch being the mainstream,while

20、 recombinant strains should be monitored and guarded against.一帶一路國際生豬產業大會（2）PoRV：A為主要流行群，呈上升態勢(2)PoRV:A is the main epidemic group,showing an upward trendn基因群：PoRV A 為主，C和B亦可檢出Gene group:PoRV A is dominant,C and B are also detectable哈獸研2018年報道RVC G6P6Harbin Veterinary Research Institute reported RVC

21、 G6P6 in 2018n血清型：PoRV A至少9個以上G型，以G9、G5、G4、G3占優勢Serotypes:PoRV A has at least 9 G types,with G9,G5,G4,and G3 predominating 年份 Year檢出率（%）Detection rate(%)PEDVTGEVRVPRV2011年Year 201194.606.4011.252012年Year 201267.822.459.852013年Year 201317.600.294.4517.452014年Year 201418.641.123.7510.422015年Year 20153

22、5.297.029.9212.002016年Year 201671.196.90注：數據來自動物衛生與疫病流行病學中心Note:Data from Animal Health and Disease Epidemiology Center一帶一路國際生豬產業大會p2012-2018年：PoRV檢出率1%以內2012-2018:PoRV detection rate less than 1%Zhang et al.BMC Veterinary Research，2019一帶一路國際生豬產業大會p江蘇農業科學院Jiangsu Academy of Agricultural Sciences2017

23、-2019年間，華東5個?。ńK、安徽、上海、浙江、山東），35個豬場，594個樣本（肛拭子、小腸組織）From 2017 to 2019,594 samples(anal swabs and small intestine tissues)were collected from 35 pig farms in 5 provinces in East China(Jiangsu,Anhui,Shanghai,Zhejiang and Shandong)區域差異較大Large regional differences哺乳仔豬和育肥豬陽性檢出率高High positive detection ra

24、te of nursery piglets and fattening pigs優勢血清型：G9P23、G9P7Dominant serotypes:G9P 23,G9P 7Ran Tao et al一帶一路國際生豬產業大會p 我們的調查 Our Investigation調查日期：2020年8月至今Investigation date:August 2020 to present調查樣本：22個養殖集團，203個豬場，1297份仔豬腹瀉病料Survey sample:22 pig farming companies,203 pig farms,1297 piglet diarrhea dis

25、ease data調查方法：RT-qPCRInvestigation method:RT-qPCR指指標標IndicatorsPEDVRVAPDCoVTGEVPEDV+RVAPEDV+RVA+PDCoV豬豬場場陽陽性性率率Positive rate of pig farms62.56%（127/203）51.23%（104/203）9.85%（20/203）1.48%（3/203）25.62%（52/203）2.96%（6/203）病病料料陽陽性性率率Positive rate of diseased material44.26%（574/1297）34.77%（451/1297）3.39%（

26、44/1297）0.54%（7/1297）/一帶一路國際生豬產業大會p 病毒類型：4種病毒均可檢出；其中PEDV 陽性率最高，其次為RV。Virus Type:All four viruses were detected;PEDV had the highest positive rate,followed by RV.p 感感染染模模式式：以單一感染為主，存在多病原混合（PEDV+RV）感染。Infection Mode:Single infection is the main type of infection,but mixed infection with multiple patho

27、gens(PEDV+RV)also occurs.p 流流行行現現狀狀：Epidemiological status:PEDV 仍是主要病原PEDV is still the main pathogenRV呈上升態勢RV is rising混合感染不容忽視Mixed infections cannot be ignored流調結果Epidemiological investigation results61.7%23.5%59.6%47.1%48.9%21.3%40.8%17.6%0.0%10.0%20.0%30.0%40.0%50.0%60.0%70.0%PEDVTGEVPEDV+PoRV2

28、1-23年腹瀉類病毒檢測（場陽性率）66.4%PoRVPDCoV2021年2022年2023年一帶一路國際生豬產業大會p 基于VP7基因分析（86個豬場，104個毒株）Based on VP7 gene analysis(86 farms,104 strains)血清型：9個 G型，呈多樣性。Serotypes:9 G types,showing diversity.優勢血清型：G9、G5、G3和G4。Predominant serotype:G9,G5,G3 and G4.p 基于VP4基因分析（42個豬場，51個毒株）Based on VP4 gene analysis(42 farms,

29、51 strains)血清型：5個 P型，呈多樣性，。Serotypes:5 P-types,showing diversity,.優勢血清型：P13、P23、P6Dominant serotypes:P13,P23,P6PoRV A 血清型：以 G9、G5、G3G4為主，同豬場G型呈多樣性PoRV A serotype:Mainly G9,G5,G3/G4,with G types showing diversity in the same farm一帶一路國際生豬產業大會PoRV 進化分析PoRV evolution analysisG5G3G9G4一帶一路國際生豬產業大會G9型：VP7氨

30、基酸同源性 95%的毒株占比90%。Type G9:90%of the strains have VP7 amino acid homology 95%.G5型：VP7氨基酸同源性 95%的毒株占比77%，同源性在88-92%占比13%。Type G5:77%of the strains have VP7 amino acid homology 95%,and 13%have homology between 88-92%.G3、G4型：大部分毒株VP7氨基酸同源性在95%以上。Types G3 and G4:The VP7 amino acid homology of most strain

31、s is over 95%.G9、G3、G4型毒株相對穩定保守；G5型毒株間差異較大。G9,G3 and G4 strains are relatively stable and conservative;G5 strains are quite different.PoRV G型同源性分析 Homology analysis of PoRV G type一帶一路國際生豬產業大會同G型間中和效果最佳The best neutralization effect is the same as G type不同G型間存在一定交叉中和性Different G types:There is a cert

32、ain degree of cross neutralizationG9對G5有較好中和性，對G3中和一般；G9 has good neutralization to G5 and fair neutralization to G3;G5對G9存在交叉中和性，對G3 中和性一般；G5 is cross-neutralizing to G9 and generally neutralizing to G3;G3對G5有較好中和性，對G9中和性一般。G3 has good neutralization to G5 and average neutralization to G9.G5型免疫原性最好

33、？G5 has the best immunogenicity?G型間交叉中和性分析 G-type intercross neutralization analysis毒株Virus strain抗血清AntiserumYY株Strain YY（G9型）(Type G9)GY株GY strain（G5型）(G5 type)GY株GY strain（G3型）(Type G3)YY株（G9型）YY strain(G9 type)579348713444GY株（G5型）GY strain(G5 type)8192231715793GY株（G3型）GY strain(G3 type)289620484

34、871一帶一路國際生豬產業大會（3）PDCoV：小范圍散發，需警惕！(3)PDCoV:Spreading in a small area,please be vigilant!p PDCoV只有一個血清型，兩個相對獨立分支，我國屬于群。PDCoV has only one serotype and two relatively independent branches,which belong to group I in China.一帶一路國際生豬產業大會（4）TGEV：檢出率低于2%，暫時忽略！(4)TGEV:The detection rate is less than 2%,ignor

35、e it for now!0.63%1.01%1.56%0.52%2019年2020年2021年2022年1-4月n數據來自華中農業大學Data from Huazhong Agricultural University一帶一路國際生豬產業大會3、圓環：PCV2占優，PCV3抬頭！3.PCV:PCV2 is dominant,PCV3 is on the rise!一帶一路國際生豬產業大會3、圓環：PCV2+PRRSV混合感染，不容忽視！3.PCV:PCV2+PRRSV mixed infection cannot be ignored!一帶一路國際生豬產業大會3、圓環：PCV3呈上升趨勢，病

36、毒及其致病性尚未定論！3.PCV:PCV3 is on the rise,and the virus and its pathogenicity have not yet been determined!-PCV3：2015年，美國PCV3:2015,US-優勢基因型：PCV3aDominant genotype:PCV3a一帶一路國際生豬產業大會4、豬瘟：免疫穩定，偶有點發！4.Classic swine fever:The immune system is stable,with occasional fever!警惕：2.1d亞群Alert:2.1d subpopulation一帶一路國

37、際生豬產業大會5、口蹄疫：總體平穩，O型為主，CATHY再現！5.Foot-and-mouth disease:Overall stable,mainly O-type,CATHY reappears!O/Mya-98O/CATHAYO/Pan AsiaO/Ind-2001東南亞拓撲型（SEA型）Southeast Asia topological type(SEA type)中東-南亞拓撲型Middle East-South Asia topology（ME-SA型）(Type ME-SA)中國古典拓撲型Chinese classical topological type（CATHAY型）(

38、Type CATHAY)一帶一路國際生豬產業大會 2024年4月19日中國動物疫病預防控制中心 疾控防【2024】50號 April 19,2024 Chinese Center for Animal Disease Control and Prevention No.50 2024 for Disease Control and Prevention一帶一路國際生豬產業大會p時間：2023年6月2024年1月Time:June 2023 to January 2024p區域：江西、湖南、湖北、廣東、內蒙古、河南Region:Jiangxi,Hunan,Hubei,Guangdong,Inne

39、r Mongolia,Henanp豬群類型：仔豬、保育豬、育肥豬、后備種豬、母豬Type of pig herd:piglets,nursery pigs,fattening pigs,reserve breeding pigs,sowsp數量：8255份Quantity:8255p樣本類型：咽拭子、肛拭子、臍帶血、病料、血液、糞便Sample type:Throat swab,anal swab,umbilical cord blood,disease material,blood,stoolp檢測內容Test content咽拭子：鏈球菌（SS）、副豬嗜血桿菌（HPS）、胸膜肺炎放線桿菌（

40、APP）、巴氏桿菌（PM）、波氏桿菌（Bb)、豬丹毒（Er）的種屬和血清型；Throat swab:species and serotypes of Streptococcus(SS),Haemophilus parasuis(HPS),Actinobacillus pleuropneumoniae(APP),Pasteurella multocida(PM),Bordetella bordetella(Bb),and swine erysipelas(Er);肛拭子：鏈球菌（SS）、大腸桿菌（Ecoli）、沙門氏菌（Se）和胞內勞森菌（LI）種屬和血清型。Anal swabs:Strepto

41、coccus(SS),Escherichia coli(Ecoli),Salmonella(Se),and Lawsonia intracellularis(LI)species and serotypes.6、細菌類：鏈球副豬仍是主流，警惕APP、APP和 LI！6.Bacteria:SS and HPS is still the mainstream,beware of APP,APP and LI!一帶一路國際生豬產業大會p5888份咽拭子檢測結果Test results of 5888 throat swabs339227299171402844557.6%46.3%15.6%2.4%

42、0.5%7.6%0.0%10.0%20.0%30.0%40.0%50.0%60.0%70.0%05001000150020002500300035004000鏈球副豬傳胸巴氏波氏丹毒咽咽拭拭子子細細菌菌病病原原種種屬屬檢檢測測數數據據Detection data of bacterial pathogenic species in throat swabs陽性數量陽性率一帶一路國際生豬產業大會血血清清型型檢檢測測結結果果Serotype test resultsS.S：各各日日齡齡豬豬群群中中均均能能檢檢到到，S.S:detectable in herds of all ages,主主流流血血

43、清清型型為為29、9、2；The mainstream serotypes are 29,9,and 2;3026017313782516523151%8%5%4%24%49%9%0%10%20%30%40%50%60%04008001200160020001或14237929其他血清型鏈鏈球球菌菌血血清清型型檢檢出出率率Serotype detection rate of Streptococcus檢出數量檢出比例223160299841806941323677550788%6%11%3%7%25%5%13%3%19%0%0%5%10%15%20%25%30%0100200300400500

44、6007008001245或1278131415未定型9型副副豬豬血血清清型型檢檢出出率率Detection rate of HPS serotypes陽性數量比例HPS：在在各各日日齡齡豬豬群群中中均均能能檢檢到到，HPS:It can be detected in pigs of all ages.主主流流血血清清型型為為8、14、4；The mainstream serotypes are 8,14 and 4;一帶一路國際生豬產業大會APP：22-35日日齡齡及及75-120日日齡齡檢檢出出率率較較高高；APP:The detection rate is higher at 22-35

45、 days and 75-120 days of age;主主流流血血清清型型7、8、2型型 Mainstream serotypes 7,8,210844617265112346713081.1%9.2%5.0%0.1%0.8%28.9%12.2%0.3%0.4%0.7%7.7%33.6%0.0%5.0%10.0%15.0%20.0%25.0%30.0%35.0%40.0%05010015020025030035012345789101115未定型APP血血清清型型檢檢出出率率Detection rate of APP serotype陽性數量比例1%0%25%25%3%8%0%5%10%1

46、5%20%25%30%0-21日齡22-35日齡36-80日齡75-210日齡母豬未標注日期豬豬丹丹毒毒不不同同日日齡齡檢檢出出率率Detection rate of swine erysipelas at different agesEr，3680日日齡齡和和75210日日齡齡檢檢出出率率相相對對較較高高Er,the detection rate is relatively high at 36-80 days and 75-210 days一帶一路國際生豬產業大會病病原原種種類類Pathogen type樣樣本本數數量量Number of samples陽陽性性數數量量Number of

47、positives陽陽性性率率Positive rate豬豬鏈鏈球球菌菌Streptococcus suis236739217%大大腸腸桿桿菌菌E.Coli2367158367%沙沙門門Salmonella23671396%胞胞內內勞勞森森菌菌L.Intracellularis2367109746%肛肛拭拭子子常常見見細細菌菌檢檢測測結結果果Test results of common bacteria in anal swabs總體檢出率：大腸桿菌、胞內勞森菌、豬鏈球菌、沙門氏菌檢出率分別為：67%、46%、17%、6%。Overall detection rate:The detectio

48、n rates of Escherichia coli,Lawsonia intracellularis,Streptococcus suis and Salmonella were 67%,46%,17%and 6%,respectively.肛拭子鏈球菌：主要血清型為29型、9型、3型；Anal swab streptococcus:The main serotypes are 29,9 and 3;大腸桿菌：主要血清型為F41/K88、987、F18/K99血清型Escherichia coli:Main serotypes are F41/K88,987,F18/K99胞內勞森菌：各日

49、齡檢出率在52%82%之間，尤其是22-35日齡檢出率最高L.Intracellularis:The detection rate is between 52%and 82%at each age,especially at 22-35 days.3921583139109717%67%6%46%0%10%20%30%40%50%60%70%80%020040060080010001200140016001800鏈球大腸沙門胞內勞森菌肛肛拭拭子子細細菌菌檢檢出出率率Bacteria detection rate of anal swabs陽性數量陽性率p2367份肛肛拭子檢測結果Test re

50、sults of 2367 anorectal swabs一帶一路國際生豬產業大會基因型Genotype血清型Serotype宿主Host嗜性Tropism季節性Seasonality培養Culture特性Characteristics致病性Pathogenicity變變異異Variation正確理解病原變異Correct understanding of pathogenic variation一帶一路國際生豬產業大會三三流行病學的臨床價值Clinical value of epidemiology一帶一路國際生豬產業大會查明真兇Identify the real culprit弄清傳播途徑

51、和流行路線Clarify transmission routes and epidemic routes指導生物安全Guidance on biosafety指導疫苗研發和臨床應用Guide vaccine development and clinical application預警疫病風險Early warning of disease risk制定聯防聯控策略Formulate joint prevention and control strategies流行病學的臨床價值Clinical value of epidemiology一帶一路國際生豬產業大會p實驗用豬：2月齡健康豬，頸部肌肉

52、注射免疫；Experimental pigs:Healthy pigs at 2 months of age,immunized by neck intramuscular injection;p免疫程序：多種免疫組合（序貫免疫）Immunization program:multiple immunization combinations(Sequential Immunization)p評價指標：中和抗體Evaluation indicator:Neutralizing antibodyp初步結論：活+滅+滅組中和抗體水平最高；滅活苗需2次免疫Preliminary conclusion:T

53、he neutralizing antibody level was the highest in the live+inactivated+inactivated group;the inactivated vaccine required 2 immunizations免疫程序Immunization ProcedurePEDV血清中和抗體（Log2)Serum Neutralizing Antibody to PEDV(Log2)RV血清中和抗體（Log2)RV serum neutralizing antibody(Log2)免前Before immunization免后21d21d

54、after immunization免后42d42d after immunization免后63d63d after immunization免前Before immunization免后21d21d after immunization免后42d42d after immunization免后63d63d after immunization活+滅+滅Live+Inactive+Inactive1.5 1.9 5.8 8.1 48.91010.8滅+滅Inactive+Inactive/0.6 2.6 7.0/4.41110.8活+活Live+Live/0.5 3.0 6.0/68.58.

55、3活/滅+滅（商品化)Live/inactivated+inactivated(commercial)/0.8 2.6 7.8/4.88.48.7攻毒對照Challenge control1.7 1.0 2.0 1.6 3.56.05.55.3空白對照Blank control1.00.751.751.53.86.55.04.5nPEDV-RV二聯苗的實驗室免疫效果評價（中和抗體）Laboratory Evaluation of Immune Effect of PEDV-RV Combined Vaccine(Neutralizing Antibody)流調背景清晰下的臨床價值Clinica

56、l value of clear epidemiological background一帶一路國際生豬產業大會p豬場背景：廣西某養殖場，母豬存欄5000頭，發生腹瀉，病原檢測僅RVA陽性，經測序確定G9型；Pig farm background:In a farm in Guangxi,diarrhea occurred in 5,000 sows in the herd.The pathogen was only RVA positive,and G9 was determined by sequencing;p疫苗制備：分離病毒、效價優化、制備RVA G9滅活疫苗，經安全性檢驗后，臨床免疫

57、；Vaccine preparation:Isolate the virus,optimize the titer,prepare the RVA G9 inactivated vaccine,and perform clinical immunity after safety test;p免疫方式：母豬產前6周、產前3周各免疫一次，1頭份/2mll頭Immunization method:Sows were immunized once at 6 weeks and 3 weeks before delivery,respectively,1 dose/2ml/headp免疫評價指標：Imm

58、une evaluation indicators:腹瀉率：免疫RVA G9滅活疫苗后，再產仔豬腹瀉發病率小于1%（豬場老板自述）。Diarrhea rate:After immunization with RVA G9 inactivated vaccine,the incidence of diarrhea in re-delivered piglets was less than 1%(as reported by the farm owner).中和抗體：初乳、母豬血清、仔豬血清Neutralizing antibodies:colostrum,sow serum,piglet ser

59、um排毒率：肛拭子,熒光定量PCR檢測，均未檢出。Detoxification rate:None was detected in anal swabs by fluorescence quantitative PCR.采集時間Collection time初乳中和抗體Colostrum neutralizing antibody（Log2）母豬血清Sow serum中和抗體（Log2）Neutralizing Antibody(Log2)母豬肛拭子檢測Sow anal swab test仔豬血清Piglet serum中和抗體（Log2）Neutralizing antibodies(Log

60、2)仔豬肛拭子檢測Anal swab test of piglets抗體值Antibody value離散度Degree of dispersion抗體值Antibody value離散度Degree of dispersion抗體值Antibody value離散度Degree of dispersion產仔當日Day of farrowing11.712.8%/產仔7日Day 7 of farrowing/7.610.2%0/107.54 10.6%0/15產仔14日Day 14 of farrowing/7.017.5%0/107.520.7%0/15nPEDV-RV（G9型）二聯滅活苗

61、豬場臨床評價（中和抗體）Clinical evaluation of pig farms with PEDV-RV(G9)bivalent inactivated vaccine(neutralizing antibody)一帶一路國際生豬產業大會p豬場背景：廣西某養殖場，PEDV、RV、PDCoV均可檢出；其中RV 為G9型；Pig farm background:A breeding farm in Guangxi,PEDV,RV,and PDCoV can all be detected;RV is G9 type;p疫苗制備：選擇與該豬場流行毒株同源性大于95%的種毒、制備三聯苗，經安

62、全性檢驗后，臨床免疫；抗原量（LgTCID50/頭份）：PEDV 7.0、PoRV 7.8、PDCoV 7.3Vaccine preparation:Select the seed virus with homology greater than 95%with the epidemic strain of the pig farm,prepare the triple vaccine,and conduct clinical immunization after safety test;antigen amount(LgTCID50/dose):PEDV 7.0,PoRV 7.8,PDCoV

63、 7.3p試驗分組：4家疫苗，平行對比；Trial grouping:Vaccines from 4 hospitals,parallel comparison;p免疫劑量：免疫后背母豬（130日齡），經PEDV活毒馴化后1個月免疫，1頭份/2ml頭；Immunization dose:The sows(130 days old)after immunization were immunized 1 month after acclimatization with live PEDV,1 dose/2ml/head;p樣品采集：免疫前、免疫后每隔1周采血一次，連續采樣4次；Sample col

64、lection:Blood samples were collected before immunization and every 1 week after immunization for 4 consecutive times;p評價指標：血清中和抗體。Evaluation indicators:Serum neutralizing antibodies.nPEDV-RV（G9型）-PDCoV三聯滅活苗的豬場臨床評價Clinical evaluation of PEDV-RV(G9)-PDCoV triple inactivated vaccine in pig farms一帶一路國際生

65、豬產業大會耳號Ear tag檢驗編號Test No.采樣日期與RV(G9)中和抗體效價Sampling date and RV(G9)neutralizing antibody titer2023.12.202024.01.032024.01.092024.01.182024.01.25129570651:641:2561:2561:10241:2048129570961:641:2561:2561:5121:512129571071:161:2561:2561:10241:1024129571181:161:2561:2561:10241:2048pRV（G9）中和抗體效價RV(G9)neu

66、tralizing antibody titer一帶一路國際生豬產業大會耳號Ear tag檢驗編號Test No.采樣日期與PEDV中和抗體效價Sampling date and PEDV neutralizing antibody titer2023.12.202024.01.032024.01.092024.01.182024.01.25129570651:321:1281:1281:5121:51212957096161:1281:2561:2561:128129571071:161:1281:321:1281:128129571181:321:321:321:5121:256pPEDV

67、中和抗體PEDV neutralizing antibody一帶一路國際生豬產業大會耳號Ear tag檢驗編號Test No.采樣日期與PDCoV中和抗體效價Date of Sampling and PDCoV Neutralizing Antibody Titer2023.12.202024.01.032024.01.092024.01.182024.01.25129570651:21:81:81:2561:128129570961:21:81:81:1281:16129571071:21:81:81:1281:32129571181:21:81:81:321:16pPDCoV 中和抗體效價

68、PDCoV neutralizing antibody titer一帶一路國際生豬產業大會樣品信息Sample Information實驗室編號Lab No.鏈球菌(SS)Streptococcus(SS)胸膜肺炎放線桿菌(APP)Actinobacillus pleuropneumoniae(APP)結果判定Judgment of results血清型Serotype結果判定Judgment of results血清型Serotype免疫前Before immunization1陽性Positive3、9、29型3.Type 3,9,29陽性Positive32陽性Positive7、9、2

69、9型7.Type 7,9,29陰性Negative/3陽性Positive3、29型3.29 type陰性Negative/4陽性Positive9、29型9.Type 9 and 29陽性Positive35陽性Positive29型Type 29陰性Negative/6陽性Positive7、29型7.Type 7 and 29陰性Negative/7陽性Positive7、29型7.Type 7 and 29陰性Negative/8陽性Positive3、9、29型3.Type 3,9,29陽性Positive39陽性Positive29型Type 29陰性Negative/10陽性Po

70、sitive3、29型3.29 type陽性Positive3免疫豬Immunized pigs11陽性Positive2、9、29型2.Type 2,9,29陰性Negative/12陽性Positive2型Type 2陽性Positive未定型Undifferentiated13陰性Negative/陰性Negative/14陽性Positive2、9陰性Negative/15陽性Positive2、9陰性Negative/16陰性Negative/陰性Negative/17陽性Positive2、9陰性Negative/18陰性Negative/陰性Negative/19陰性Negati

71、ve/陰性Negative/20陰性Negative/陰性Negative/nS.S-APP-PM三聯六價滅活疫苗的臨床評價Clinical Evaluation of S.S-APP-PM Triple Hexavalent Inactivated Vaccine注注：APP：1、7；PM：D；S.S：3、7、29Note:APP:1,7;PM:D;S.S:3,7,29一帶一路國際生豬產業大會免前血清抗體效價檢測Serum antibody titer test before immunization二免前血清抗體檢測Serum antibody test before the second

72、 immunization編號No.APP1型Type APP1APP7型Type APP7編號No.APP1型Type APP1APP7型Type APP711:21:411:161:1621:21:221:161:1631:21:831:81:1641:21:241:161:851:21:251:161:861:21:261:161:871:21:271:321:1681:21:281:321:891:41:891:161:32101:21:2101:161:32陽性Positive1:128陽性Positive陽性Positive1:128陽性Positive陰性Negative1:2陰

73、性Negative陰性Negative1:2陰性Negative備注：1、陰性血清判定：抗體效價應不高于1:4。Note:1.Determination of negative serum:The antibody titer should be no more than 1:4.nAPP（1、7型）抗體轉陽率檢測Detection of APP(type 1 and 7)antibody positive conversion rate一帶一路國際生豬產業大會四四流行病學的未來挑戰Future challenges of epidemiology一帶一路國際生豬產業大會p 總策略：病原流調+

74、生物安全+疫苗免疫+免疫檢測 General strategy:Pathogen epidemiological regulation+biosafety+vaccine immunization+immune detection防控前移：病原流調+生物安全+養殖設備+環境凈化+養殖模式+選址Forward prevention and control:pathogen epidemiological investigation+biosafety+pig farming equipment+environmental purification+pig farming mode+site se

75、lection病原監測：基因變異、抗原性變異、致病力變異、中和性Pathogen monitoring:gene variation,antigenicity variation,pathogenicity variation,neutralization疫苗免疫：免疫程序、序貫免疫Vaccine immunization:immunization procedures,Sequential Immunization免疫檢測：群體免疫合格率、離散度；個體抗體陽性率、抗體滴度等Immune detection:Herd immunity qualification rate and disper

76、sion;individual antibody positive rate and antibody titer,etc.n防控策略；疫苗+Prevention and control strategies;Vaccines+一帶一路國際生豬產業大會p 思想觀念不更新Thoughts and concepts are not updatedp 調研數據不共享 Research data is not sharedp 調研數據不動態 The survey data is not dynamicp 生物安全不平衡 Unbalanced biosecurityp 疫苗免疫不精準 Vaccine i

77、mmunity is inaccuratep 技術創新不及時 Technological innovation is not timelyp 疫病凈化不落實 Epidemic purification not implementedFuture challenges一帶一路國際生豬產業大會疫病防控，不過度依賴疫苗Epidemic prevention and control,without excessive reliance on vaccines不減少死亡No/reduced mortality癥狀無、輕或RV陽性穩定No or mild symptoms or stable RV pos

78、itive不感染、不排毒，不散毒No infection,no detoxification,no toxin dispersion無疫情發生No epidemic occurred疫病凈化Epidemic Purification一帶一路國際生豬產業大會4個個領領域域4 areas國國家家規規程程指指標標Indicators of national regulations臨臨床床免免疫疫指指標標Clinical immune indicators生生產產關關鍵鍵指指標標Key Indicators of Production經經濟濟效效益益指指標標Economic benefit index

79、抗原含量Antigen content外觀性狀Appearance and properties安檢Security check效檢Efficacy test粒徑Particle size抗體轉陽率Antibody conversion rate抗體離散度Dispersion of antibody抗體滴度Antibody titer抗體離散度Dispersion of antibody群體免疫合格率Qualified rate of herd immunity整體死淘率Overall dead panning rate整體均勻度Overall uniformity呼吸道發生率Respirat

80、ory Incidence日增重Daily weight gain料肉比Ratio of raw materials to meat上市天數Days on the market精細化與數字化Refinement and digitization疫苗評價，不過度依賴抗體Vaccine evaluation,without excessive reliance on antibodies不僅是抗體Not only antibodies帶毒率Virus-carrying rate病毒血癥Viraemia病毒載量Viral load一帶一路國際生豬產業大會傳染源Source of infection易

81、感動物Susceptible animal傳播途徑Route of transmission降降低低Decrease減減少少Decrease消消滅滅Elimination營營養養Nutrition免免疫疫Immunization選選址址Site selection消消毒毒Disinfection隔隔離離Quarantine切切斷斷Cut off生物安全，不平衡Biosafety,unbalanced一帶一路國際生豬產業大會敬請各位領導、專家批評指正！Please criticize and correct leaders and experts!謝謝！Thank you!致謝：感謝所有引用數據單位及合作單位！Acknowledgements:Thank all referenced data units and cooperative units!敬請批評指正！Please criticize and correct!謝謝！Thank you!一帶一路國際生豬產業大會