唐麗杰-2011年至今我國PEDV變異株與其他腹瀉病毒共感染的分子流行病學調查與分析.pdf

《唐麗杰-2011年至今我國PEDV變異株與其他腹瀉病毒共感染的分子流行病學調查與分析.pdf》由會員分享，可在線閱讀，更多相關《唐麗杰-2011年至今我國PEDV變異株與其他腹瀉病毒共感染的分子流行病學調查與分析.pdf（43頁珍藏版）》請在三個皮匠報告上搜索。

1、2 20 01 11 1年年至至今今我我國國P PE ED DV V變變異異株株與與其其他他腹腹瀉瀉病病毒毒共共感感染染的的分分子子流流行行病病學學調調查查與與分分析析唐麗杰 教授 博士生導師 Professor Tang Lijie PhD Advisor東北農業大學 Northeast Agricultural University2024年4月26日 April 26,2024Investigation of transmission and evolution of PEDV variants and co-infections in China over past decades一帶

2、一路國際生豬產業大會仔豬腹瀉概況 Introduction of diarrhea in piglets仔豬腹瀉是引起仔豬生長受阻和仔豬高死亡率的重要原因,疾病的防治一直是一個引人注目的世界性問題。Diarrhea in piglets is an important disease causing growth restriction and high mortality,and its the prevention and treatment has always been a global issue.養豬人最恐懼的疫情之一。One of the most feared outbreak

3、s by pig farmers.3日齡以內仔豬感染死亡率可達100%，7日齡以內發病豬死亡率達50%80%。The mortality rate of piglets infected within 3 days of age can reach 100%,and the mortality rate of pigs infected within 7 days of age can reach 50%to 80%.豬腹瀉病一直是困擾養豬業重要疫病，也是影響養豬業的重要疾病之一。Swine diarrhea has always been an important disease that

4、troubles the pig farming industry and is also one of the important diseases affecting the industry.2011年至今，豬腹瀉病高發，PEDV單獨感染及多病原混合感染在規?；B殖場流行，可能也與生豬養殖貿易的全球化有關，推動了新病原或新的變異病原出現和傳播。Since 2011,there has been a high incidence of porcine diarrhea disease,with the prevalence of PEDV single infection and mixe

5、d infection of multiple pathogens in large-scale pig farms.This may also be related to the globalization of pig farming trade,which has promoted the emergence and spread of new or mutated pathogens.一帶一路國際生豬產業大會病病毒毒名名稱稱S Sp pe ec ci ie es s種種類類g ge en no om me e科科屬屬F Fa am mi il ly y G Ge en nu us s有

6、有無無囊囊膜膜E En nv ve el lo op pe e我我國國首首次次報報道道年年份份F Fi ir rs st t r re ep po or rt te ed d i in n C Ch hi in na aC Cl li in ni ic ca al l s si ig gn ns s臨臨床床特特征征豬豬流流行行性性腹腹瀉瀉病病毒毒（P PE ED DV V）R RN NA A冠冠狀狀病病毒毒科科甲甲型型冠冠狀狀病病毒毒屬屬有有囊囊膜膜1 19 98 84 4年年嘔嘔吐吐、水水樣樣腹腹瀉瀉和和脫脫水水新新生生仔豬發發病病死死亡亡率率為為8 80 0%-1 10 00 0%V Vo

7、 om mi it ti in ng g,w wa at te er ry y d di ia ar rr rh he ea a,a an nd d d de eh hy yd dr ra at ti io on nT Th he e m mo or rt ta al li it ty y r ra at te e o of f n ne ew wb bo or rn n p pi ig gl le et ts s i is s 8 80 0%-1 10 00 0%豬豬傳傳染染性性胃胃腸腸炎炎病病毒毒 (T TG GE EV V)R RN NA A 冠冠狀狀病病毒毒科科甲甲型型冠冠狀狀病病毒毒

8、屬屬有有囊囊膜膜1 19 95 56 6 年年與與P PE ED DV V相相似似S Si im mi il la ar r t to o P PE ED DV V豬豬輪輪狀狀病病毒毒 (P PR Ro oV V)R RN NA A 呼呼腸腸孤孤病病毒毒科科輪輪狀狀病病毒毒屬屬無無囊囊膜膜1 19 98 81 1 年年厭食、嘔吐、下痢豬群發病率16%-40%Anorexia,vomiting,and dysenteryIncidence rate of pig herd:16%-40%豬豬捷捷申申病病毒毒（P PT TV V）R RN NA A 微微R RN NA A病病毒毒科科捷捷申申病病毒

9、毒屬屬無無囊囊膜膜2 20 00 03 3年年豬豬腦腦脊脊髓髓炎炎、母母豬豬繁繁殖殖障障礙礙、肺肺炎炎、腹腹瀉瀉、心心包包炎炎和和心心肌肌炎炎P Po or rc ci in ne e e en nc ce ep ph ha al lo om my ye el li it ti is s,r re ep pr ro od du uc ct ti iv ve e d di is so or rd de er rs s i in n s so ow ws s,p pn ne eu um mo on ni ia a,d di ia ar rr rh he ea a,p pe er ri ic ca

10、ar rd di it ti is s,a an nd d m my yo oc ca ar rd di it ti is s 引起豬腹瀉的病毒種類（一）Classfication of Viruses causing dirrhea(I)一帶一路國際生豬產業大會 引起豬腹瀉的病毒種類（二）Classfication of Viruses causing dirrhea(II)病病毒毒名名稱稱S Sp pe ec ci ie es s種種類類g ge en no om me e科科屬屬F Fa am mi il ly y G Ge en nu us s有有無無囊囊膜膜E En nv ve el

11、 lo op pe e我我國國首首次次報報道道年年份份F Fi ir rs st t r re ep po or rt te ed d i in n C Ch hi in na aC Cl li in ni ic ca al l s si ig gn ns s臨臨床床特特征征豬豬腸腸道道病病毒毒G G（P PE EV V）R RN NA A微微R RN NA A病病毒毒科科腸腸病病毒毒屬屬無無囊囊膜膜2 20 00 03 3年年繁繁殖殖障障礙礙、腹腹瀉瀉、脊脊髓髓灰灰質質炎炎R Re ep pr ro od du uc ct ti iv ve e d di is so or rd de er

12、rs s,d di ia ar rr rh he ea a,p po ol li io om my ye el li it ti is s豬豬星星狀狀病病毒毒（P PA AS St tV V）R RN NA A微微R RN NA A病病毒毒科科哺哺乳乳動動物物星星狀狀病病毒毒屬屬無無囊囊膜膜2 20 00 06 6年年嚴嚴重重腹腹瀉瀉S Se ev ve er re e d di ia ar rr rh he ea a豬豬嵴嵴病病毒毒（P PK KV V）R RN NA A微微R RN NA A病病毒毒科科嵴嵴病病毒毒屬屬無無囊囊膜膜2 20 00 09 9年年嚴嚴重重腹腹瀉瀉、病病毒毒血血癥

13、癥S Se ev ve er re e d di ia ar rr rh he ea a,a an nd d v vi ir re em mi ia a豬豬托托克克特特諾諾病病毒毒2 2型型（T TT TS Su uV V-2 2）豬豬細細環環病病毒毒D DN NA A細細環環病病毒毒科科指指環環病病毒毒屬屬無無囊囊膜膜2 20 00 09 9年年與與多多種種病病毒毒協協同同致致病病C Co ol ll la ab bo or ra at ti in ng g w wi it th h m mu ul lt ti ip pl le e v vi ir ru us se es s t to o

14、c ca au us se e d di is se ea as se e一帶一路國際生豬產業大會 近年來新發的腹瀉病毒（三）New diarrhea virus emerging in recent years(III)病病毒毒名名稱稱S Sp pe ec ci ie es s種種類類g ge en no om me e科科屬屬F Fa am mi il ly y G Ge en nu us s有有無無囊囊膜膜E En nv ve el lo op pe e我我國國首首次次報報道道年年份份F Fi ir rs st t r re ep po or rt te ed d i in n C Ch

15、 hi in na aC Cl li in ni ic ca al l s si ig gn ns s臨臨床床特特征征豬豬德德爾爾塔塔冠冠狀狀病病毒毒 (P PD DC Co oV V)R RN NA A冠冠狀狀病病毒毒科科丁丁型型冠冠狀狀病病毒毒屬屬有有囊囊膜膜2 20 01 12 2年年哺哺乳乳仔仔豬豬嘔嘔吐吐、腹腹瀉瀉、脫脫水水、昏昏睡睡N Nu ur rs si in ng g p pi ig gl le et ts s v vo om mi it ti in ng g,d di ia ar rr rh he ea a,d de eh hy yd dr ra at ti io on n

16、,l le et th ha ar rg gy y豬豬薩薩佩佩羅羅病病毒毒（P PS SV V）R RN NA A 微微R RN NA A病病毒毒科科薩薩佩佩羅羅病病毒毒屬屬無無囊囊膜膜2 20 01 11 1年年腹腹瀉瀉、脊脊髓髓灰灰質質炎炎、肺肺炎炎和和繁繁殖殖障障礙礙D Di ia ar rr rh he ea a,p po ol li io om my ye el li it ti is s,p pn ne eu um mo on ni ia a,a an nd d r re ep pr ro od du uc ct ti iv ve e d di is so or rd de er

17、rs s豬豬博博卡卡病病毒毒 (P PB Bo oV V)D DN NA A細細小小病病毒毒科科博博卡卡病病毒毒屬屬無無囊囊膜膜2 20 01 10 0年年仔仔豬豬多多系系統統衰衰竭竭綜綜合合征征、呼呼吸吸綜綜合合征征及及腹腹瀉瀉M Mu ul lt ti is sy ys st te em m f fa ai il lu ur re e s sy yn nd dr ro om me e,r re es sp pi ir ra at to or ry y s sy yn nd dr ro om me e,a an nd d d di ia ar rr rh he ea a i in n p p

18、i ig gl le et ts s在在眾眾多多的的豬豬腹腹瀉瀉病病毒毒中中，對對我我國國生生豬豬養養殖殖行行業業影影響響最最大大的的仍仍然然是是P PE ED DV VA Am mo on ng g t th he e n nu um me er ro ou us s p pi ig g d di ia ar rr rh he ea a v vi ir ru us se es s,P PE ED DV V s st ti il ll l h ha as s t th he e g gr re ea at te es st t i im mp pa ac ct t o on n C Ch hi

19、 in na a s s p pi ig g b br re ee ed di in ng g i in nd du us st tr ry y一帶一路國際生豬產業大會引發嘔吐、腹瀉和脫水，發病率高，死亡率可達 100%。It causes vomiting,diarrhea and dehydration,with high morbidity and mortality of up to 100%.豬流行性腹瀉病毒（Porcine epidemic diarrhea virus,PEDV）冠狀病毒科，甲型冠狀病毒屬Coronaviridae,the genus Anacoronavirus

20、豬流行性腹瀉病毒是如何傳播和發展的？什么原因導致其在近些年的大規模爆發？PEDV的流行和發展歷程How does the porcine epidemic diarrhea virus spread and develop?What has caused its massive explosion in recent years?Epidemic and progression of PEDV一帶一路國際生豬產業大會豬流行性腹瀉的流行歷程The epidemic course of PED1971年，英格蘭首次暴發1971，England first outbreak1983年，日本報道流行1

21、983，epidemic reported in Japan1973年，中國首次報告1973，first reported in China2006年，免疫豬群傳播2006，outbreak in immunized herd日本在1993-1994年期間暴發的PED疫情就導致 14 000 頭豬死亡，哺育仔豬的死亡率為 30%100%；1996 年冬季，108 個豬場發生PED，哺育仔豬約70%死亡率（56256/39509）。隨后PEDV在亞洲開始廣泛傳播。The outbreak of PED in Japan during 1993-1994 resulted in 14 000 pi

22、gs,and the mortality rate of feeding piglets was 30%to 100%;in the winter of 1996,108 farms experienced PED,feeding piglets by about 70%(56256/39509).PEDV then began to spread widely in Asia.一帶一路國際生豬產業大會我國將其列為二類動物疫病。第一階段：1971年至2010年初呈地方性流行第二階段：2010年-至今 世界范圍內爆發1971年至2010年PEDV在我國流行部分地區示意圖2010年-至今 PEDV

23、部分流行地區示意圖P PE ED D給全球養豬業造成了嚴重的經濟損失，成為危害養豬業的重要疫病。P PE ED D h ha as s c ca au us se ed d s se er ri io ou us s e ec co on no om mi ic c l lo os ss se es s t to o t th he e g gl lo ob ba al l p pi ig g i in nd du us st tr ry y,a an nd d h ha as s b be ec co om me e a an n i im mp po or rt ta an nt t d

24、di is se ea as se e t th ha at t h ha ar rm ms s t th he e p pi ig g i in nd du us st tr ry y.豬流行性腹瀉的流行歷程The epidemic course of PED一帶一路國際生豬產業大會豬流行性腹瀉病毒生物學特性 基因突變導致新基因型出現推動新的爆發和流行，也是病毒逃避免疫的關鍵因素之一。Mutations leading to the emergence of new genotypes drive new outbreaks and epidemics,and are also one of

25、 the key factors for the virus to evade immunity.編碼S蛋白的S基因在PEDV流行性毒株的遺傳變異和流行過程中發揮重要的作用。The S gene encoding the S protein plays an important role in the genetic variation and prevalence of PEDV epidemic strains.一帶一路國際生豬產業大會豬流行性腹瀉病毒的S蛋白The S protein of porcine epidemic diarrhea virus一帶一路國際生豬產業大會PEDV的S

26、基因與病毒流行的關系T Th he e R Re el la at ti io on ns sh hi ip p b be et tw we ee en n t th he e S S GGe en ne e o of f P PE EDDV V a an nd d V Vi ir ru us s E Ep pi id de emmi ic cP PE ED DV V流流行行毒毒株株不不同同亞亞型型的的占占比比S Su u M M,e et t a al l.A A m mo ol le ec cu ul la ar r e ep pi id de em mi io ol lo og gi i

27、c ca al l i in nv ve es st ti ig ga at ti io on n o of f P PE ED DV V i in n C Ch hi in na a:C Ch ha ar ra ac ct te er ri iz za at ti io on n o of f c co o-i in nf fe ec ct ti io on n a an nd d g ge en ne et ti ic c d di iv ve er rs si it ty y o of f S S1 1-b ba as se ed d g ge en ne es s.T Tr ra an

28、 ns sb bo ou un nd da ar ry y a an nd d e em me er rg gi in ng g d di is se ea as se es s.2 20 01 19 9跨跨國國貿貿易易與與P PE ED DV V在在世世界界范范圍圍內內的的傳傳播播關關系系Z Zh ha an ng g H H,e et t a al l.G Gl lo ob ba al l D Dy yn na am mi ic cs s o of f P Po or rc ci in ne e E En nt te er ri ic c C Co or ro on na av vi ir

29、 ru us s P PE ED DV V E Ep pi id de em mi io ol lo og gy y,E Ev vo ol lu ut ti io on n,a an nd d T Tr ra an ns sm mi is ss si io on n.M Mo ol l B Bi io ol l E Ev vo ol l.2 20 02 23 3一帶一路國際生豬產業大會PEDV S蛋白的55-64殘基對G1毒株（經典毒株）具有特異性識別的能力，而157-164 的殘基對G2毒株的具有特異性。Residues 55 64 of the PEDV S protein have th

30、e ability to specifically recognize the G1 strain(the classical strain),while residues 157 164 are specific for the G2 strain.（Zhang H,et al.Global Dynamics of Porcine Enteric Coronavirus PEDV Epidemiology,Evolution,and Transmission.Mol Biol Evol.2023）PEDV不斷進化，G2型毒株的爆發和流行給PEDV有效防控帶來挑戰。PEDV continues

31、 to evolve,and the outbreak and prevalence of G2 strains bring challenges to the effective prevention and control of PEDV.PEDV的S基因與病毒流行的關系T Th he e R Re el la at ti io on ns sh hi ip p b be et tw we ee en n t th he e S S GGe en ne e o of f P PE ED DV V a an nd d V Vi ir ru us s E Ep pi id de emmi ic

32、 c與CV777毒株相比，2011年后流行毒株因 S 基因存在堿基缺失/插入及基因突變現象，使新流行毒株與之前毒株出現明顯差異；導致病毒致病力、傳染性及免疫原性發生顯著變化。Compared with CV777 strain,the new epidemic strain caused the S strain,which resulted in its pathogenicity,infectivity and immunogenicity.PEDV流行毒株的分子流行病學調查，對疾病的防控和疫苗的研發具有重要意義。Molecular epidemiological investigatio

33、n of circulating PEDV strains is of great significance for disease prevention and control and vaccine research and development.一帶一路國際生豬產業大會 豬流行性腹瀉病毒的共感染情況華華東東地地區區2017-2019年年的的豬豬腹腹瀉瀉病病混混合合感感染染情情況況（常常新新見見,等等.20172019年年華華東東地地區區豬豬場場主主要要病病毒毒性性腹腹瀉瀉病病原原調調查查.畜畜牧牧獸獸醫醫學學報報,2020）河河南南地地區區豬豬腹腹瀉瀉病病混混合合感感染染情情況況（Zh

34、ang YF,et al.Isolation and Characterization of Porcine Sapelovirus from the PDCoV-Positive Sample and Its Molecular Epidemiology in Henan Province,China.Transboundary and Emerging Diseases.2023）安安徽徽、北北京京、福福建建等等中中國國22個個省省市市豬豬腹腹瀉瀉病病混混合合感感染染情情況況（Su M,et al.A molecular epidemiological investigation of P

35、EDV in China:Characterization of co-infection and genetic diversity of S1-based genes.Transboundary and emerging diseases.2019）Co-infection of PEDV with multiple pathogens in piglets with diarrhea一帶一路國際生豬產業大會對對2 20 01 15 5-2 20 02 22 2年年黑黑龍龍江江省省，吉吉林林省省，遼遼寧寧省省，內內蒙蒙古古自自治治區區3 32 27 7份份仔仔豬豬腹腹瀉瀉病病料料進進行行病

36、病毒毒性性病病原原鑒鑒定定。3 32 27 7份份病病料料中中1 12 2種種腹腹瀉瀉病病毒毒的的臨臨床床檢檢出出率率C Cl li in ni ic ca al l d de et te ec ct ti io on n r ra at te e o of f 1 12 2 d di ia ar rr rh he ea a v vi ir ru us se es s i in n 3 32 27 7 s sa am mp pl le es s樣品數量0100一帶一路國際生豬產業大會氨基酸的共有突變模式v2015年至2022年間仔豬PEDV陽性樣品中經測序得到了155株流行毒株的S基因；收集了

37、2011年-2022年世界范圍內流行的PEDV毒株的S 基因共計732 個。S genes from 155 circulating strains were obtained in piglets PEDV positive samples between 2015 and 2022;732 S genes from PEDV strains circulating worldwide between 2011 and 2022.一帶一路國際生豬產業大會S S5 58 8：Q QG GN NV V，S S1 15 56 6：H H/Y Y，S S3 35 59 9：N NM MR RS S的

38、的插插入入；S S3 32 28 8 S S3 35 58 8 S S3 36 66 6 S S7 77 72 2 S S7 77 76 6 S S7 78 82 2 S S8 81 14 4 S S9 90 04 4 S S9 96 67 7 S S9 97 73 3 S S9 98 81 1 S S1 10 03 32 2 S S1 10 05 52 2 S S1 11 17 75 5 S S1 12 20 01 1 S S1 12 24 41 1 S S1 12 26 68 8 S S1 13 30 06 6的的替替換換；S S1 12 20 02 2的的缺缺失失構構成成了了流流行行毒毒

39、株株的的共共有有突突變變模模式式。v2015年至2022年間仔豬PEDV陽性樣品中經測序得到了155株流行毒株的S基因；收集了2011年-2022年世界范圍內流行的PEDV毒株的S 基因共計732 個。突變位點包括：病毒的唾液酸活性位點、RBD區域，抗原表位區，可能是病毒產生免疫逃逸進而流行爆發的關鍵性位點。氨基酸的共有突變模式一帶一路國際生豬產業大會S S5 58 8：Q QG GN NV V，S S1 15 56 6：H H/Y Y，S S3 35 59 9：N NM MR RS S的的插插入入；S S3 32 28 8 S S3 35 58 8 S S3 36 66 6 S S7 77

40、72 2 S S7 77 76 6 S S7 78 82 2 S S8 81 14 4 S S9 90 04 4 S S9 96 67 7 S S9 97 73 3 S S9 98 81 1 S S1 10 03 32 2 S S1 10 05 52 2 S S1 11 17 75 5 S S1 12 20 01 1 S S1 12 24 41 1 S S1 12 26 68 8 S S1 13 30 06 6的的替替換換；S S1 12 20 02 2的的缺缺失失構構成成了了流流行行毒毒株株的的共共有有突突變變模模式式。v2015年至2022年間仔豬PEDV陽性樣品中經測序得到了155株流行

41、毒株的S基因；收集了2011年-2022年世界范圍內流行的PEDV毒株的S 基因共計732 個。突變位點包括：病毒的唾液酸活性位點、RBD區域，抗原表位區，可能是病毒產生免疫逃逸進而流行爆發的關鍵性位點。PEDV的進化趨勢是什么樣的呢？What is the evolutionary trend of PEDV?氨基酸的共有突變模式一帶一路國際生豬產業大會將將逐逐年年流流行行的的P PE ED DV V毒毒株株與與經經典典毒毒株株C CV V7 77 77 7進進行行比比對對發發現現，所所有有的的流流行行毒毒株株氨氨基基酸酸突突變變主主要要集集中中在在S S1 1 區區域域，且且氨氨基基酸酸突

42、突變變率率不不斷斷增增高高。Comparing the popular PEDV strain with the classic strain CV777,it was found that all popular strains had amino acid mutations mainly concentrated in the S1 region,and the amino acid mutation rate continued to increase.近年來P PE ED DV V流行毒株氨基酸突變統計分析一帶一路國際生豬產業大會年年份份新新增增突突變變位位點點2 20 01 12

43、2Y Y2 29 93 3H H、Q Q3 31 17 7P P、T T3 37 73 3I I、I I5 50 05 5T T、S S5 57 76 6T T、K K6 63 39 9I I、T T8 83 33 3I I、V V8 85 52 2 A A、S S9 97 74 4 A A、P P1 12 27 74 4S S2 20 01 13 3L L5 51 10 0 P P2 20 01 14 4Q Q8 89 98 8K K2 20 01 15 5V V5 55 56 6A A、R R1 14 45 5L L2 20 01 16 6L L5 59 9 S S、T T3 36 65

44、5I I、T T4 49 96 6R R、P P5 50 04 4S S、I I5 50 05 5T T、P P7 77 72 2S S、V V8 85 52 2A A、S S9 97 74 4A A、Q Q1 10 06 67 7K K2 20 01 17 7I I3 32 25 5T T、S S5 55 58 8T T、A A8 83 34 4S S、V V1 13 34 43 3A A2 20 01 18 8P P1 12 27 74 4S S2 20 01 19 9L L2 27 71 1 V V、A A2 28 88 8T T M M2 28 89 9T T、N N4 45 51 1

45、T T、S S5 52 26 6T T、V V8 88 86 6A A、S S9 97 74 4A A、A A1 10 09 95 5S S、A A1 12 26 63 3S S2 20 02 20 0G G2 29 96 6V V2 20 02 21 1D D3 32 27 7A A2 20 02 22 2S S9 97 74 4A AS S蛋白氨基酸逐年新增，且新增數量具有一定的周期性，2 20 01 12 2年，2 20 01 16 6年和2 20 01 19 9年新增數量最多，其余年份較少。T Th he e a am mi in no o a ac ci id ds s o of f

46、 S S p pr ro ot te ei in n i in nc cr re ea as se ed d y ye ea ar r b by y y ye ea ar r,a an nd d t th he e n nu um mb be er r o of f n ne ew w a ac ci id ds s h ha ad d a a c ce er rt ta ai in n p pe er ri io od di ic ci it ty y.I In n 2 20 01 12 2,2 20 01 16 6 a an nd d 2 20 01 19 9,t th he e n nu

47、 um mb be er r o of f n ne ew w a ac ci id ds s w wa as s t th he e l la ar rg ge es st t,w wh hi il le e t th he e o ot th he er r y ye ea ar rs s w we er re e l le es ss s.一帶一路國際生豬產業大會ACRecombination analysisDistribution of recombination events in prevalent non-Chinese PEDV strains.重重組組事事件件在在主主要要非

48、非中中國國PEDV毒毒株株中中的的分分布布。Recombination event distribution of prevalent PEDV strains in Chinese geographic regions.Association of recombination events of PEDV strains prevalent in non-Chinese and Chinese regions.非非中中國國和和中中國國地地區區流流行行的的PEDV毒毒株株重重組組事事件件的的關關聯聯。中國流行毒株中，廣東和四川發生重組事件的概率較高 Among Chinese circulat

49、ing strains,the probability of recombination events was higher in Guangdong and Sichuan廣東流行株與韓國和墨西哥流行株關系密切，四川流行株與日本、越南和墨西哥流行株關系更為密切。Guangdong epidemic strains are closely related to Korea and Mexico,and Sichuan epidemic strain is more closely related to Japan,Vietnam and Mexico.1 18 8/1 16 68 87 75

50、5/1 16 68 8B重組分析可揭示P PE ED DV V的起源、分布及其在不同地理位置和時間段的進化途徑R Re ec co om mb bi in na at ti io on n a an na al ly ys si is s c ca an n r re ev ve ea al l t th he e o or ri ig gi in n,d di is st tr ri ib bu ut ti io on n o of f P PE ED DV V a an nd d i it ts s e ev vo ol lu ut ti io on na ar ry y p pa at

51、th hw wa ay y i in n d di if ff fe er re en nt t g ge eo og gr ra ap ph hi ic c l lo oc ca at ti io on ns s a an nd d t ti im me e p pe er ri io od ds s一帶一路國際生豬產業大會ACRecombination analysisDistribution of recombination events in prevalent non-Chinese PEDV strains.Recombination event distribution of p

52、revalent PEDV strains in Chinese geographic regions.Association of recombination events of PEDV strains prevalent in non-Chinese and Chinese regions.1 18 8/1 16 68 87 75 5/1 16 68 8B重組分析可揭示P PE ED DV V的起源、分布及其在不同地理位置和時間段的進化途徑R Re ec co om mb bi in na at ti io on n a an na al ly ys si is s c ca an n

53、r re ev ve ea al l t th he e o or ri ig gi in n,d di is st tr ri ib bu ut ti io on n o of f P PE ED DV V a an nd d i it ts s e ev vo ol lu ut ti io on na ar ry y p pa at th hw wa ay y i in n d di if ff fe er re en nt t g ge eo og gr ra ap ph hi ic c l lo oc ca at ti io on ns s a an nd d t ti im me e

54、 p pe er ri io od ds s不同地區毒株重組的位置是否存在差異？Are there differences in the location of PEDV strain recombination between different regions?中國流行毒株中，廣東和四川發生重組事件的概率較高 Among Chinese circulating strains,the probability of recombination events was higher in Guangdong and Sichuan廣東流行株與韓國和墨西哥流行株關系密切，四川流行株與日本、越南和墨

55、西哥流行株關系更為密切。Guangdong epidemic strains are closely related to Korea and Mexico,and Sichuan epidemic strain is more closely related to Japan,Vietnam and Mexico.一帶一路國際生豬產業大會P PE ED DV V 流行毒株S S基因重組位置的數據統計Statistics on the recombination positions of prevalent PEDV strains from 2011 to 2022.1-1444bp 144

56、5-2888bp 2889-4149bpP PE ED DV V 重組更為普遍，中國流行的毒株重組速度比其他國家和地區快，重組毒株與韓國和越南流行的毒株關系密切。P PE ED DV V r re ec co om mb bi in na at ti io on n i is s m mo or re e p pr re ev va al le en nt t,t th he e s st tr ra ai in ns s c ci ir rc cu ul la at ti in ng g i in n C Ch hi in na a r re ec co om mb bi in ne e f

57、 fa as st te er r t th ha an n o ot th he er r c co ou un nt tr ri ie es s a an nd d r re eg gi io on ns s,a an nd d t th he e r re ec co om mb bi in na an nt t s st tr ra ai in ns s a ar re e c cl lo os se el ly y r re el la at te ed d t to o t th he e s st tr ra ai in ns s c ci ir rc cu ul la at t

58、i in ng g i in n K Ko or re ea a a an nd d V Vi ie et tn na am m.一帶一路國際生豬產業大會2 20 01 11 1-2 20 02 22 2年P PE ED DV V流行毒株遺傳進化分析G Ge en ne et ti ic c E Ev vo ol lu ut ti io on n A An na al ly ys si is s o of f P PE ED DV V E Ep pi id de em mi ic c S St tr ra ai in ns s f fr ro om m 2 20 01 11 1 t to o

59、2 20 02 22 2G GI Ia a亞亞群群：6 6株株（3 3.8 87 7%）G GI Ib b亞亞群群：0 0株株（0 0%）G GI II Ia a亞亞群群：1 1株株（0 0.6 64 4%）G GI II Ib b亞亞群群：1 13 39 9株株（8 89 9.6 67 7%）重重組組毒毒株株：9 9株株（5 5.8 80 0%）G GI II Ib b亞亞群群毒毒株株逐逐年年增增加加G GI II Ia a亞亞群群毒毒株株逐逐年年減減少少G GI II Ib b亞亞群群毒毒株株的的防防控控壓壓力力逐逐漸漸增增加加東 北 地 區 所 鑒 定 的155株PEDV樣品中：揭示了P

60、 PE ED DV V的遺傳多樣性和復雜性，表明P PE ED DV V在不同地區和不同時期存在變異和重組。R Re ev vc co ov ve er rs s t th he e g ge en ne et ti ic c d di iv ve er rs si it ty y a an nd d c co om mp pl le ex xi it ty y o of f P PE ED DV V,i in nd di ic ca at ti in ng g v va ar ri ia at ti io on n a an nd d r re ec co om mb bi in na at

61、 ti io on n i in n P PE ED DV V i in n d di if ff fe er re en nt t r re eg gi io on ns s a an nd d t ti im me e p pe er ri io od ds s.一帶一路國際生豬產業大會(l l)2 20 01 11 1_ _和和2 20 01 12 2_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白的的比比較較分分析析(a a)C CV V7 77 77 7株株和和2 20 02 22 2_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的

62、比比較較分分析析(b b)2 20 02 21 1_ _和和2 20 02 22 2_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白的的比比較較分分析析(c c)2 20 02 20 0_ _和和2 20 02 21 1_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(d d)2 20 01 19 9_ _和和2 20 02 20 0_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(e e)2 20 01 18 8_ _和和2 20 01 19 9_ _P PE ED DV V株株

63、之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(f f)2 20 01 17 7_ _和和2 20 01 18 8_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(g g)2 20 01 16 6_ _和和2 20 01 17 7_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(h h)2 20 01 15 5_ _和和2 20 01 16 6_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(i i)2 20 01 14 4_ _和和2 20

64、01 15 5_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(j j)2 20 01 13 3_ _和和2 20 01 14 4_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白質質的的比比較較分分析析(k k)2 20 01 12 2_ _和和2 20 01 13 3_ _P PE ED DV V株株之之間間預預測測的的S S-蛋蛋白白的的比比較較分分析析Comparative analysis of S-protein models of popular PEDV strains from 2011-2022以CV777毒

65、株的S蛋白位模板將2011-2022年的流行毒株S蛋白進行了建模比對，發現PEDV流行毒株的S蛋白結構逐年發生變化。We compared the epidemic strain S protein from 2011-2022 using the S protein bit template of CV777 strain and found that the S protein structure of the PEDV epidemic strain changes year by year.一帶一路國際生豬產業大會P PE ED DV V流流行行株株S S蛋蛋白白模模型型結結構構變變化

66、化位位點點統統計計S St ta at ti is st ti ic ca al l a an na al ly ys si is s o of f s st tr ru uc ct tu ur ra al l c ch ha an ng ge es s i in n t th he e S S p pr ro ot te ei in n m mo od de el l o of f P PE ED DV V e ep pi id de em mi ic c s st tr ra ai in ns s發現2011-2022所流行毒株在S蛋白的NTD蛋白結構逐年變化區域和在RBD區域內逐年變化結

67、構位置可能是病毒進化和發展的方向；2019年前后結構變化最少，這一現象可能與2019年的低檢出率相關聯。S蛋白NTD的aa55-64、aa113-117、aa133附近、aa198附近蛋白結構逐年變化，該區域可能是PEDV流行毒株傳播能力變化的關鍵區域。在RBD區域內aa520及aa553附近結構逐年變化，該區域可能是PEDV流行毒株侵染能力變化的關鍵區域。It is found that the circulating strains in 2011-2022 may be the direction of the virus evolution and development;the le

68、ast around 2019,and this phenomenon may be associated with the low detection rate in 2019.The S protein protein structure of aa 55-64,aaa 113-117,and aa 198 change year by year,and this region may be the key region for the change in the transmission ability of the PEDV epidemic strain.In the RBD,the

69、 structure around aa 520 and aa 553 changes year to year,and this region may be the key area for changes in the infection ability of the PEDV epidemic strain.一帶一路國際生豬產業大會1 1.2 20 01 11 1-2 20 02 22 2所所流流行行的的P PE ED DV V毒毒株株在在S S蛋蛋白白的的N NT TD D a aa a5 55 5-6 64 4、a aa a1 11 13 3-1 11 17 7、a aa a1 13

70、 33 3附附近近、a aa a1 19 98 8附附近近蛋蛋白白結結構構逐逐年年變變化化。2 2.在在R RB BD D區區域域內內a aa a5 52 20 0及及a aa a5 55 53 3附附近近結結構構逐逐年年變變化化。一帶一路國際生豬產業大會1 1.2 20 01 15 5-2 20 02 22 2年年我我國國東東北北地地區區豬豬腹腹瀉瀉病病料料檢檢測測結結果果表表明明，近近年年來來P PE ED DV V是是腹腹瀉瀉病病的的主主要要病病原原之之一一。1 1.R Re es su ul lt ts s i in n N No or rt th he ea as st t C Ch

71、 hi in na a f fr ro om m 2 20 01 15 5 t to o 2 20 02 22 2 i in nd di ic ca at te e t th ha at t P PE ED DV V h ha as s b be ee en n o on ne e o of f t th he e m ma ai in n p pa at th ho og ge en ns s o of f d di ia ar rr rh he ea a.2 2.P PE ED DV V流流行行毒毒株株在在S S蛋蛋白白的的突突變變上上存存在在一一定定的的共共有有突突變變模模式式。2 2.

72、T Th he e P PE ED DV V e ep pi id de em mi ic c s st tr ra ai in ns s h ha as s a a c ce er rt ta ai in n c co om mm mo on n m mu ut ta at ti io on n p pa at tt te er rn n i in n t th he e S S p pr ro ot te ei in n.3 3.流流行行的的P PE ED DV V毒毒株株中中G GI II Ib b亞亞群群毒毒株株的的比比例例在在增增加加，而而G GI II Ia a亞亞群群毒毒株株的的

73、比比例例從從2 20 01 11 1年年到到2 20 02 22 2年年逐逐年年下下降降，說說明明現現階階段段G GI II Ib b亞亞群群毒毒株株的的防防控控壓壓力力逐逐漸漸增增加加。3 3.T Th he e n nu um mb be er r o of f P PE ED DV V G GI II Ib b s st tr ra ai in ns s i is s i in nc cr re ea as si in ng g,w wh hi il le e t th ha at t o of f P PE ED DV V G GI II Ia a s st tr ra ai in n

74、s s i is s d de ec cr re ea as si in ng g y ye ea ar rl ly y f fr ro om m 2 20 01 11 1 t to o 2 20 02 22 2,i in nd di ic ca at ti in ng g t th ha at t G GI II Ib b s st tr ra ai in ns s a ar re e i in nc cr re ea as si in ng gl ly y p pr re ev va al le en nt t.4 4.P PE ED DV V流流行行毒毒株株在在S S蛋蛋白白N NT T

75、D D的的a aa a5 55 5-6 64 4、a aa a1 11 13 3-1 11 17 7、a aa a1 13 33 3、a aa a1 19 98 8附附近近蛋蛋白白結結構構逐逐年年變變化化，上上述述區區域域可可能能是是P PE ED DV V流流行行毒毒株株傳傳播播能能力力變變化化的的關關鍵鍵區區域域，在在R RB BD D區區域域的的a aa a5 52 20 0及及a aa a5 55 53 3附附近近結結構構逐逐年年變變化化，可可能能是是P PE ED DV V流流行行毒毒株株侵侵染染能能力力變變化化的的關關鍵鍵因因素素。4 4.Y Ye ea ar rl ly y c

76、ch ha an ng ge es s i in n N NT TD D o of f a aa a5 55 5-6 64 4,a aa a1 11 13 3-1 11 17 7,a aa a1 13 33 3,a an nd d a aa a1 19 98 8 i in n S S p pr ro ot te ei in n m ma ay y b be e r re es sp po on ns si ib bl le e f fo or r t th he e t tr ra an ns sm mi is ss si io on n a ab bi il li it ty y o of

77、f P PE ED DV V e ep pi id de em mi ic c s st tr ra ai in ns s,w wh hi il le e c co on ns st ta an nt t c ch ha an ng ge es s n ne ea ar r a aa a5 52 20 0 a an nd d a aa a5 55 53 3 i in n t th he e R RB BD D r re eg gi io on n m ma ay y b be e r re el la at te ed d t to o i in nf fe ec ct ti io on n

78、a ab bi il li it ty y o of f P PE ED DV V e ep pi id de em mi ic c s st tr ra ai in ns s.小小結結一帶一路國際生豬產業大會一帶一路國際生豬產業大會01003 32 27 7份份樣樣品品中中1 12 2種種腹腹瀉瀉病病毒毒在在黑黑、吉吉、遼遼、內內蒙蒙古古自自治治區區的的空空間間分分布布S Sp pa at ti ia al l d di is st tr ri ib bu ut ti io on n o of f 1 12 2 d di ia ar rr rh he ea a v vi ir ru us s

79、e es s i in n 3 32 27 7 s sa am mp pl le es s i in n H He ei i,J Ji i,L Li ia ao o a an nd d I In nn ne er r M Mo on ng go ol li ia a a au ut to on no om mo ou us s r re eg gi io on ns s檢檢出出數數量量一帶一路國際生豬產業大會3 32 27 7份份樣樣品品中中1 12 2種種腹腹瀉瀉病病毒毒的的單單獨獨感感染染率率及及所所占占比比重重S Se ep pa ar ra at te e i in nf fe ec

80、ct ti io on n r ra at te e a an nd d p pr ro op po or rt ti io on n o of f 1 12 2 d di ia ar rr rh he ea a v vi ir ru us se es s i in n 3 32 27 7 s sa am mp pl le es sP PE ED DV V：7 70 0.9 9%P Po oR RV V：3 32 2.7 72 2%P PT TV V：3 30 0.2 28 8%P PC CV V：2 29 9.0 05 5%P PA As st tV V：1 12 2.8 84 4%P PK

81、 KV V：6 6.7 72 2%T TG GE EV V：6 6.4 42 2P PS SV V：3 3.9 98 8P PE EV V ：3 3.3 36 6%一帶一路國際生豬產業大會1 12 2種種腹腹瀉瀉病病毒毒的的混混合合感感染染率率T Th he e m mi ix xe ed d i in nf fe ec ct ti io on n r ra at te e o of f 1 12 2 d di ia ar rr rh he ea a v vi ir ru us se es s66.97%的樣本含有至少2種病原體，39.75%的樣本含有3 5種不同的病原體66.97%of th

82、e samples contain at least 2 pathogens,and 39.75%of t h e s a m p l e s c o n t a i n 3-5 different pathogensP PE ED DV V與與其其他他1 11 1種種腹腹瀉瀉病病毒毒的的共共感感染染率率C Co oi in nf fe ec ct ti io on n r ra at te e o of f P PE ED DV V w wi it th h 1 11 1 o ot th he er r d di ia ar rr rh he ea a v vi ir ru us se es

83、 s其其他他1 11 1種種腹腹瀉瀉病病毒毒與與P PE ED DV V的的共共感感染染率率C Co o i in nf fe ec ct ti io on n r ra at te es s o of f 1 11 1 o ot th he er r d di ia ar rr rh he ea a v vi ir ru us se es s w wi it th h P PE ED DV V232份PEDV陽性樣品中，PRoV(40.08%)、PTV(34.91%)和PCV(23.32%)混合感染最多Of the 232 PEDV-positive samples,PRoV(40.08%)

84、,PTV(34.91%),and PCV(23.32%)had the most mixed infections3 32 27 7 份份樣樣品品中中，P PK KV V和和P PR Ro oV V與與P PE ED DV V的的混混合合感感染染率率最最高高Among 327 samples,PKV and PRoV had the highest mixed infection rate with PEDVABC一帶一路國際生豬產業大會2 20 01 15 5-2 20 02 22 2年年3 32 27 7份份樣樣品品中中1 12 2種種腹腹瀉瀉病病毒毒逐逐年年檢檢出出情情況況A An nn

85、 nu ua al l d de et te ec ct ti io on n o of f 1 12 2 t ty yp pe es s o of f d di ia ar rr rh he ea a v vi ir ru us se es s i in n 3 32 27 7 s sa am mp pl le es s f fr ro om m 2 20 01 15 5 t to o 2 20 02 22 2豬豬捷捷申申病病毒毒豬豬嵴嵴病病毒毒豬豬薩薩佩佩羅羅病病毒毒豬豬腸腸道道病病毒毒豬豬博博卡卡病病毒毒豬豬托托克克特特諾諾病病毒毒豬豬星星狀狀病病毒毒一帶一路國際生豬產業大會在在黑黑、吉

86、吉、遼遼和和內內蒙蒙古古自自治治區區新新出出現現P PS SV V、P PT TV V、P PA As st tV V、P PK KV V等等致致豬豬腹腹瀉瀉的的病病毒毒。P PS SV V,P PT TV V,P PA As st tV V,P PK KV V a an nd d o ot th he er r n ne ew w s sw wi in ne e d di ia ar rr rh he ea a-c ca au us si in ng g v vi ir ru us se es s a ap pp pe ea ar re ed d i in n H He ei il lo o

87、n ng gj ji ia an ng g,J Ji il li in n,L Li ia ao on ni in ng g a an nd d I In nn ne er r M Mo on ng go ol li ia a a au ut to on no om mo ou us s r re eg gi io on ns s.一帶一路國際生豬產業大會P PS SV V的的致致病病性性及及變變異異分分析析P Pa at th ho og ge en ni ic ci it ty y a an nd d V Va ar ri ia at ti io on n A An na al ly ys

88、 si is s o of f P PS SV V豬豬薩薩佩佩羅羅病病毒毒一帶一路國際生豬產業大會 薩佩羅病毒的結構模式圖 薩薩佩佩羅羅病病毒毒（PSV）臨臨床床癥癥狀狀：腹瀉、肺炎、腦脊髓灰質炎、生殖系統障礙等多系統綜合征，也可能出現無癥狀感染。Clinical symptoms:Multiple system syndromes such as diarrhea,pneumonia,poliomyelitis,reproductive system disorders,etc.,and asymptomatic infections may also occur.微RNA 病毒科薩佩羅病毒

89、屬 病毒主要通過糞-口途徑傳播MicroRNA viridae is mainly transmitted through the fecal-oral routeHLJ 2204株在PK-15細胞內繁殖達107.386 TCID50/0.1 mLThe HLJ 2204 strain was propagated in PK-15 cells up to 107.386 TCID50/0.1 mL一帶一路國際生豬產業大會2 20 01 11 1-2 20 02 22 2年P PS SV V流行毒株遺傳進化分析Genetic Evolution Analysis of PSV Epidemic

90、 Strains from 2011 to 2022東北地區所鑒定的17株PSV與Genbank上收錄的137株進行系統發育分析Phylogenetic analysis of 17 PSVs identified in Northeast China and 137 strains included in Genbank發現所有檢出的PSV序列與國內流行的毒株親緣關系較近；部分毒株與湖南、河南和甘肅流行株親緣關系最近。說明2015-2022年間我國東北地區流行的PSV毒株很有可能是由上述毒株演化而來。All detected PSV sequences were closely relate

91、d to the domestic circulating strains;some strains were closely related to Hunan,Henan and Gansu.This indicates that the PSV strains circulating in northeast China between 2015 and 2022 were most likely evolved from the above strains.一帶一路國際生豬產業大會不不同同國國家家的的PSV的的基基因因進進化化率率Gene evolution rates of PSV i

92、n different countries不不同同地地區區PSV的的逐逐年年進進化化率率Annual rate of evolution of PSV in different regions中中國國與與國國外外地地區區PSV流流行行毒毒株株總總重重組組事事件件差差異異統統計計PSV epidemic strains in China and foreign regionsDifference statistics of total recombination events共共觀觀察察到到180個個重重組組事事件件A total of 180 recombination events were

93、 observedP PS SV V流行毒株的遺傳進化分析Genetic Evolution Analysis of PSV Epidemic Strains顯示PSV在韓國正經歷著最快速的進化 Showed that PSV is undergoing the most rapid evolution in Korea在未來的進化過程中，印度地區的流行毒株可能會擁有更高的進化率，進而產生更多樣性的病毒種群。In future evolution,endemic strains in India may have higher rates of evolution,which will the

94、n produce more diverse virus populations.？一帶一路國際生豬產業大會國國內內外外PSV流流行行毒毒株株重重組組率率差差異異Differences in the recombination rates of the PSV epidemic strains at home and abroad國國內內外外PSV流流行行毒毒株株重重組組情情況況Recombination of the PSV epidemic strains at home and abroadAB中國流行毒株中沒有外國毒株參與重組的事件數是127件，其次是韓國16件，國內重組最少的是南非只

95、有1件。The number of events without foreign strains involved in recombination was 127,followed by 16 in South Korea and one in South Africa.不同的流行地域是否存在重組差異呢？Are there any restructuring differences in the different endemic regions?P PS SV V流行毒株的遺傳進化分析Genetic Evolution Analysis of PSV Epidemic Strains一帶一

96、路國際生豬產業大會國國外外流流行行的的PSV毒毒株株與與國國內內毒毒株株重重組組事事件件統統計計Statistics of recombination events of foreign circulating PSV strains and domestic strains國國內內PSV流流行行毒毒株株與與國國外外毒毒株株重重組組事事件件統統計計Statistics on recombination events between domestic PSV epidemic strains and foreign strains參與了國內流行毒株的重組Involved in the recom

97、bination of domestic circulating strainsP PS SV V流行毒株的遺傳進化分析Genetic Evolution Analysis of PSV Epidemic Strains一帶一路國際生豬產業大會1 1.2 20 01 15 5年年至至今今在在我我國國東東北北三三省省和和內內蒙蒙古古自自治治區區存存在在P PE ED DV V的的大大范范圍圍流流行行。1.Since 2015,there has been a widespread outbreak of PEDV in northeast and Inner Mongolia of China.

98、2 2.多多種種腹腹瀉瀉病病毒毒與與P PE ED DV V共共感感染染增增加加了了腹腹瀉瀉疾疾病病防防控控難難度度。2.The co infection of multiple diarrhea viruses with PEDV increases the difficulty of controlling diarrhea.3 3.近近年年來來國國內內外外流流行行的的P PE ED DV V毒毒株株S S蛋蛋白白存存在在大大規規模模突突變變的的同同時時也也存存在在一一定定的的共共有有突突變變模模式式，部部分分區區域域的的突突變變規規律律可可能能對對疾疾病病防防控控和和疫疫苗苗研研發發具具

99、有有重重要要意意義義。3 3.Recently,the S protein of PEDV strains,both domestically and internationally,exhibits both large-scale mutations and certain shared mutation patterns,which may be of great significance for disease control and vaccine development.4 4.以以P PS SV V為為代代表表的的的的豬豬腹腹瀉瀉病病毒毒在在國國內內逐逐漸漸流流行行，基基因因進進

100、化化研研究究表表明明，該該病病毒毒存存在在大大規規模模突突變變和和大大范范圍圍流流行行的的可可能能。4.The porcine diarrhea virus,represented by PSV,is gradually becoming popular in China.Genetic evolution studies have shown that the virus has the possibility of large-scale mutations and widespread spread.5 5.重重組組關關系系研研究究結結果果表表明明，世世界界范范圍圍內內的的流流行行毒毒株

101、株存存在在緊緊密密關關系系，生生豬豬進進出出口口貿貿易易推推動動著著病病毒毒的的進進化化和和發發展展。5.The research on recombination indicate that there is a close relationship between epidemic strains worldwide,and the import and export trade of live pigs is driving the evolution and development of the virus.小小 結結一帶一路國際生豬產業大會防防控控建建議議Control sugges

102、tions病病毒毒名名稱稱S Sp pe ec ci ie es s豬豬流流行行性性腹腹瀉瀉病病毒毒T TG GE EV V豬豬傳傳染染性性胃胃腸腸炎炎病病毒毒 P PE ED DV V豬豬輪輪狀狀病病毒毒 R Ro ot ta av vi ir ru us s豬豬捷捷申申病病P PT TV V豬豬薩薩佩佩羅羅病病毒毒P PS SV V豬豬腸腸道道病病毒毒P PE EV V豬豬星星狀狀病病毒毒P PA AS St tV V豬豬博博卡卡病病毒毒P PB Bo oV V豬豬托托克克特特諾諾病病毒毒2 2型型T TT TS Su uV V-2 2豬豬嵴嵴病病毒毒P PK KV V豬豬德德爾爾塔塔冠冠

103、狀狀病病毒毒P PD DC Co oV V有有無無疫疫苗苗Vaccine滅滅活活苗苗KilledT TGGE E、P PE EDD二二聯聯活活苗苗b bi iv va al le en nt t l li iv ve eP PE ED D、T TG GE E、P PR Ro oV V三三聯聯滅滅活活苗苗trivalent killed無NO無NO無NO無NO無NO無NO無NOK Ki il ll le ed d 滅滅活活苗苗流行季節endemic season冬、春季交替Winter and spring 冬季、春季Winter and spring 晚秋、冬季和早春late fall and

104、 all winter,ealry spring四季流行，深秋和初春最為嚴重four seaons with late fall and early spring being worst無明顯季節性No obvious seasonality無明顯季節性No obvious seasonality秋冬交替Fall and Winter 不詳N.A.不詳N.A.不詳N.A.冬冬季季春春季季Winter and spring 一帶一路國際生豬產業大會防防控控建建議議Control suggestions 1.完完善善豬豬場場區區域域的的功功能能劃劃分分，降降低低交交叉叉感感染染風風險險。1.Des

105、ign function areas based on pig flow and biosecurity and reduce the risk of cross infection.2.定定期期消消毒毒，做做好好環環境境空空氣氣流流通通與與凈凈化化，減減少少感感染染條條件件。2.Regular disinfection,good air circulation and decontamination,reducing pathogen load.3.保保障障豬豬舍舍適適宜宜溫溫度度、濕濕度度，減減少少應應激激對對腸腸道道產產生生刺刺激激性性腹腹瀉瀉。3.Supply ambient temp

106、erature and humidity to reduce stress induced diarrhea in the intestines.4.減減少少豬豬群群亞亞健健康康，凈凈化化疫疫病病，減減少少母母豬豬疾疾病病垂垂直直傳傳播播。4.Reduce subclincial infection,eradicate diseases,and reduce vertical transmission of diseases.疾疾病病預預防防方方面面：Prevention measures益益生生菌菌群群、抗抗病病毒毒肽肽復復合合益益生生菌菌等等微微生生態態制制劑劑有有助助于于預預防防仔仔豬豬腹腹瀉瀉病病的的發發生生！P Pr ro ob bi io ot ti ic cs s,a an nt ti iv vi ir ra al l p pe ep pt ti id de e,a an nd d p pr ro ob bi io ot ti ic cs s c ca an n h he el lp p r re ed du uc ce e p pi ig gl le et t d di ia ar rr rh he ea a!一帶一路國際生豬產業大會Thanks謝謝一帶一路國際生豬產業大會