GnRH類似物在豬定時輸精上的正確使用大衛·雷耶羅Vetoquino.pdf

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1、FTAI in Swine based on GnRH analoguesGnRH類似物在豬定時輸精上的正確使用REYERO David Vetoquinol 大衛雷耶羅 內容母豬的發情周期回顧豬FTAI的現有方案豬場的效益母豬的發情周期Oestrus cycle of the sowOestrus cycle of the sow母豬的發情周期母豬的發情周期day 115分娩farrowing斷奶weaning配種breedinglactation 14 to 28 days14-28天泌乳weaning to oestrus interval 3 to 8 days斷奶至發情間隔3-8天

2、Oestrus day 0,ovulation and fertilization.發情，排卵和受胎Uterine entry day 2 to 3 進入子宮2-3天Embryos migrate and space out day 7 to 10胚胎遷移并在第7天到第10天“分開”Maternal recognition of pregnancy day 12 to 14懷孕第12至14天Graphical representation(scale is not precise)of the reproduction time line in sows.Depending on zoo-te

3、chnical and/or selected production schemes per the preference of the producer,lactation days/weaning timing is variable but most will work between 21 to 28 days.根據生產者偏好選擇的生產方案，泌乳天數/斷奶時間是可變的，但大多數將在21至28天之間工作。母豬繁殖時間線的圖形表示15 Sow reproduction milestones.day 115farrowingweaningbreedinglactation 14 to 28

4、daysweaning to oestrus interval 3 to 8 days Oestrus day 0,ovulation and fertilization.Uterine entry day 2 to 3Embryos migrate and space out day 7 to 10Depending on zoo-technical and/or selected production schemes per the preference of the producer,lactation days/weaning timing is variable but most w

5、ill work between 21 to 28 days.On average a 21 day oestrous cycle calendar.平均有21天的發情周期。(LH)luteinizing hormone surge (heat)oestrus,ovulation LH峰值，峰值，排卵排卵First day that heat is observed is usually designated as day 0,thus in total 21 days from day 0 to day 20.觀測觀測發情發情的第一天通常被指定為第的第一天通常被指定為第0天，因此從第天，因此

6、從第0天到天到第第20天共天共21天。天。Oestrus(heat)usually lasts 24 to 72 hours.發情通常持續發情通常持續24至至72小時。小時。Ovulation normally occurs towards the end of oestrus(heat),at which time the follicles release their ova(eggs).排卵通常發生在發情結束時，此時卵泡釋放卵子。排卵通常發生在發情結束時，此時卵泡釋放卵子。Oestrus cycle of the sowOestrus cycle of the sow母豬的發情周期母豬的

7、發情周期Hypothalamic-Pituitary Gonadal Axis Diagram.下丘腦-垂體-性腺軸The interaction of“structures”and hormones in the estrous cycle.在發情周期中“結構”和激素的相互作用。6hypothalamusanterior pituitaryovaryuterusfolliclecorpus luteum Primary function of the hypothalamus is to secrete gonadotropin-releasing hormone(GnRH).下丘腦的主要功

8、能是分泌促性腺激素釋放激素(GnRH)。GnRH signals the anterior pituitary gland to release follicle-stimulating hormone(FSH)and luteinizing hormone(LH).GnRH信號垂體前葉腺釋放卵泡刺激素(FSH)和黃體生成素 FSH/LH transported by the systemic blood circulation to the ovaries,initiates various morphological changes(ex.:,follicular growth,ovula

9、tion,etc.).FSH/LH經全身血液循環運輸至卵巢，引發各種形態變化(如卵泡生長、排卵等)。Follicles containing ova(eggs),produce estrogen.卵泡含有卵子，產生雌激素。LH ruptures follicle,ovulation occurs,egg can now be fertilized.LH破裂卵泡，排卵發生，卵子現在可以受精。Corpus luteum(CL)forms on the surface of the ovary after ovulation,releasing progesterone(P4).排卵后，黃體(CL)

10、在卵巢表面形成，釋放孕酮(P4)。In the latter stage of the estrous cycle,uterine cells produce prostaglandin(PGF2),which is responsible for CL regression.在發情周期的后期，子宮細胞產生前列腺素(PGF2)，這是CL回歸的原因。At this point there are 3 hormones that interact to maintain pregnancy(if egg is fertilized),or to restart the estrous cycle.

11、此時，有3種激素相互作用來維持妊娠(如果卵子受精)，或重新啟動發情周期。Estrogen present due to the recent follicle,creates a positive feedback on the hypothalamus&anterior pituitary,which would release GnRH.由于最近的卵泡，雌激素在下丘腦和垂體前葉上產生正反饋，從而釋放GnRH。At the same time as prior,a negative feedback mechanism is created by the CL which produces

12、P4,whereby GnRH secretion is inhibited.與此同時，CL產生P4，形成負反饋機制，抑制GnRH分泌。P4 also prepares the uterus to receive fertilized egg.P4還為子宮接受受精卵做準備。When P4 reaches high enough levels,PGF2 is secreted by the uterus.當P4達到足夠高的水平時，PGF2由子宮分泌。If the egg is not fertilized,PGF2 will regress CL,ending negative feedback

13、,initiating a new cycle.如果卵子沒有受精，PGF2將回歸CL，結束負反饋，開始一個新的周期。If the egg is fertilized,there is a ND signal that comes from embryo preventing the release of PGF2 until the end of the gestation.如果卵子受精，胚胎會發出ND信號，阻止PGF2的釋放，直到妊娠結束。Oestrus cycle of the sowOestrus cycle of the sow母豬的發情周期母豬的發情周期Oestrus cycle o

14、f the sowOestrus cycle of the sow母豬的發情周母豬的發情周期期1620002010153015010201005105040302015oestrous days發情 天oestrous daysng/mlng/mlprolactinFSHLHoestradiolprogesteroneLH促黃體生成促黃體生成素素FSH促卵泡生成促卵泡生成素素oestradiol雌雌激素激素progesterone孕酮孕酮prolactin催乳素催乳素prostaglandin前列腺素前列腺素Endogenous hormonal fluctuations in normall

15、y cycling female swine.正常循環的母豬內源激素波動。Graphical representation(scale is not precise)based on average 21 day oestrous cycle.Hormones are chemical messengers providing signals at the cellular level and they have a number of functions including the timing of physiological processes within the oestrous c

16、ycle and reproduction.激素是在細胞水平上提供信號的化學信使，它們有許多功能，包括在發情周期和生殖過程中的生理過程的時間?；仡欂iFTAI的現有方案External Hormones for Ovulation purposesExternal Hormones for Ovulation purposes用于排卵的外部激素用于排卵的外部激素下丘腦垂體卵巢釋放GnRH釋放FSH/LH分泌雌激素并排卵17External Hormones for Ovulation purposesExternal Hormones for Ovulation purposes用于排卵的外部激

17、素用于排卵的外部激素Taking apart the use of products based on natural FSH and LH分開使用基于天然FSH和LH的產品ActiveDescription描述描述Administration使使用用Target effect目標效果目標效果Gonadorelin戈那瑞林GnRH agonistInjectable注射LH relase stimulation for luteinization of mature follicules instigating ovulationLH 釋放刺激成熟卵泡促進排卵Busereline布舍瑞林GnR

18、H agonistInjectable注射Triptorelin曲普瑞林GnRH agonistIntravaginal陰道用藥Hormones for Ovulation purposesHormones for Ovulation purposes用于排卵的激素 Gonadorelin戈那瑞林 Busereline布舍瑞林 Triptorelin曲普瑞林All of them have the same technical GnRH effect它們都具有GnRH效應FSH/LH ratioFSH/LH比率Administration route給藥途徑Timing of administ

19、ration用藥時間有何不同？Protocols for FT-AI in Swine豬定時輸精方案Day0WEANING斷奶斷奶Day4HEAT CHECK查查情情Day5AI-1 D4HEAT CHECK第第4天發情第天發情第1次次AI查查情情Day6AI-2 D4第第4天第天第2次次AIAI-1 D5第第5天發情，第天發情，第1次次AIHEAT CHECK查情查情Day7AI-2 D5第第5天發情第天發情第2次次AIAI-1 D6第第6天發情第天發情第1次次AIHEAT CHECK查情查情Day8AI-2 D6第第6天發情第天發情第2次次AIAI-1 D7第第7天發情第天發情第1次次AI

20、HEAT CHECK查情查情THURSDAY周周四四MONDAY周周一一TUESDAY周周二二WEDNESDAY周周三三THURSDAY周周四四FRIDAY周周五五Protocols for FT-AI in Swine定時輸精方案Day0WEANING斷奶斷奶THURSDAY周周四四Day7THURSDAY周周四四Day8FRIDAY周周五五Day1FRIDAY周周五五PMSG10:00Day4LACT.PERIOD4 WEEKS4周哺乳周哺乳MONDAY周周一一Gonadorelin戈那瑞林 10:00 LACT.PERIOD3-4 WEEKS3-4周哺乳周哺乳Gonadorelin戈那瑞

21、林 16:00 18:00 Day5TUESDAY周周二二LACT.PERIOD4 WEEKS4周哺乳周哺乳1st AI 10:00 12:00LACT.PERIOD3-4 WEEKS1st AI16:00 18:0018:00 20:00LACT.PERIOD3-4 WEEKS3-4周哺乳周哺乳2nd AI08:00Day6WEDNESDAY周周三三LACT.PERIOD4 WEEKS4周哺乳周哺乳2nd AI 02:00Protocols for FT-AI in Swine定時輸精方案Day0WEANING斷奶斷奶Day7THURSDAY周周四四Day8FRIDAY周周五五Day 4 t

22、o Day 5第第4至第至第5天天MONDAY to TUESDAY周一至周周一至周四四Busereline from Monday at 21:00 to Tuesday 03:00布舍瑞林，從周一晚21點至周二凌晨3點Day6WEDNESDAY周周三三SFT-AI單次定時輸精03:00 06:00or09:00 12:00Protocols for FT-AI in Swine母豬定精輸精方案Day0WEANING斷奶斷奶THURSDAY周周二二Day7THURSDAY周周四四Day8FRIDAY周周五五曲普照瑞林Day4MONDAY周周一一Triptorelin 10:00 Day5TU

23、ESDAY周周二二SFT-AI08:00 10:00Day6WEDNESDAY周周三三單次定時輸精豬場同步排卵的好處Benefits of Ovulation Synchronization at farm level豬場同步排卵的好處Traditional Breeding is a protocol adapted to the sow傳統配種是一種適應母豬的配種方案Fixed Time protocols are adapted to the farm定時輸精是一種適應豬場的配種方案Benefits of FTAI-OverviewFTAI的好處-概述Organizational Ben

24、efits組織層面的好處o Easy protocol to apply 便于應用的方案 o You take back control of the breeding process 掌控配種o You can reduce time and resources for heat detection 減少查情的時間和強度o Your best worker at the right time 最好的工人用在最好的時間 Production improvement or securing breeding 提高繁殖，安全配種o Improve or secure Farrowing rate 提高分娩率o Improve or secure Piglets per sow 提供更多的小豬Less semen use.important issue depending on the farm but節省精液o Genetic farms with high semen costs in pure breeds 種豬場純種精液成本高o Commercial farms with better semen quality 肉豬場可以用更好質量的精液