生物強化植物修復技術用來處理被1,4-二惡烷（有機化合物有毒）污染的地下水.pdf

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1、Reid Simmer,Emily Jansen,Joel Burken,Lou Licht,Tim Mattes,and Jerry SchnoorRemTEC&Emerging Contaminants SummitOctober 15,2024BioaugmentationCO21,4-D1,4-D1,4-D1,4-DioxaneChlorinatedSolventsBioaugmented Phytoremediation to Treat 1,4-Dioxane Contaminated GroundwaterER21-5096ER-2719Data Source:EPA UCMR3

2、UCMR3:1,4-Dioxane Contamination in Finished Drinking Water US Public Water Systems,2013-2015Simmer et al.,20202“1,4-Dioxane in drinking water:emerging for 40 years and still unregulated”3-McElroy,Hyman and Knappe,Current Opinion in Environmental Science&Health,20193ITRC,2021 EPA HRL=0.35 ppb3Source:

3、ITRChttps:/14d-1.itrcweb.org/regulatory-framework/#2_2Common remediation strategies are costlyAdvanced Oxidation Facility for Dioxane Treatment,Tucson,AZAdvanced Oxidation Facility for Dioxane Treatment,Arden Hills,MN4MetabolitesEvapotranspirationPhytoremediation is a low cost,greenremediation techn

4、ology for 1,4-dioxane mass removal5Phytoremediation is a low cost,greenremediation technology for 1,4-dioxane mass removal6“76 to 83%of the dioxane taken up by poplars was transpired from leaf surfaces”MetabolitesEvapotranspiration6ChlorinatedSolventsInterstate Technology and Regulatory Council,2021

5、MetabolitesEvapotranspiration77Bioaugmented phytoremediation speeds treatment of 1,4-dioxaneCO2MineralizationMetabolitesEvapotranspirationBioaugmentation88CO2MineralizationBioaugmentationCO2MetabolitesEvapotranspirationBioaugmentationChlorinatedSolventsBioaugmentationCO2MineralizationMetabolitesEvap

6、otranspiration1111ChlorinatedSolventsBioaugmentationCO2MineralizationMetabolitesEvapotranspiration12Limit of Detection:1 mg/L(GC/FID)12ChlorinatedSolventsBioaugmentationCO2MineralizationMetabolitesEvapotranspiration1313ER-27191314Does Mycobacterium sp.PH-06 utilize root exudates as a supplementary c

7、arbon source?Can bioaugmentation with PH-06 remove 1,4-dioxane faster than CB1190?15Pseudonocardia dioxanivorans CB1190Mycobacterium dioxanotrophicus PH-06 Root extract acted as auxiliary supplement for CB119016Root extract did not increase growth of PH-0617ChlorinatedSolventsBioaugmentationCO2Miner

8、alizationMetabolitesEvapotranspiration1818ChlorinatedSolventsBioaugmentationCO2MineralizationMetabolitesEvapotranspiration19Pseudonocardia dioxanivorans CB1190Mycobacterium dioxanotrophicus PH-06 1920Trees onlyTrees+CB1190Trees+PH-06CB1190 onlyPH-06 onlySterile Glass Rod ControlPoplar trees readily

9、removed dioxane to LOQ 2121Bioaugmenting trees with CB1190 accelerated removal2222Trees bioaugmented with Mycobacterium sp.PH-06 sped treatment of 1,4-D to 4 ug/L2323TreatmentDays to reach LOQ(4 ppb)Degradation Rate Constant(day-1)Transpiration Rate(mL day-1)Trees Only290.29 0.01332.81 2.53CB1190220

10、.37 0.034N/ATrees+CB1190190.34 0.03125.72 9.64PH-06290.23 0.015N/ATrees+PH-06130.56 0.04627.87 3.50Trees+PH-06 outpaced all other treatmentsBioaugmentation with CB1190 Removed 79%of Initial 1,4-Dioxane2525Bioaugmentation with PH06 Removed 81%of Initial 1,4-Dioxane2626Strainqmax(mg 1,4-dioxane/mg pro

11、tein/day)Ks(mg 1,4-dioxane/L)Yield(mg protein/mg dioxane)Smin(g 1,4-dioxane/L)ReferencePseudonocardia dioxanivorans CB11901.65 0.056.32 0.220.45 0.09487.14 173.45aBarajas-Rodriguez et al.(2018)Mycobacterium dioxanotrophicus PH-06Not reported78.00 10.000.16Not reportedHe et al.(2018)However,metabolic

12、 degraders have significant kinetic limitations2728Can novel metabolic dioxane-degrading strains treat low-level 1,4-dioxane(100 g/L)to below health advisory levels?Do vitamin supplements enhance metabolism of dioxane?29Vitamin B1(thiamine)supplements accelerate growth in Rhodococcus ruber 219-VB1+V

13、B1Simmer et al.,202130Vitamin B1(thiamine)supplements accelerate growth in Rhodococcus ruber 219-VB1+VB131Simmer et al.,202131Simmer et al.,202132Simmer et al.,2021Simmer et al.,2021Simmer et al.,2021R.ruber 219 repeatedly degraded 100 g/L dioxane to 0.35 g/L36036902550751001250.0000.0040.0080.012Ti

14、me(d)1,4-Dioxane(g/L)Optical Density(600 nm)1,4-DioxaneR.ruber 219 OD600Simmer et al.,2021Strainqmax(mg 1,4-dioxane/mg protein/day)Ks(mg 1,4-dioxane/L)Yield(mg protein/mg dioxane)Smin(g 1,4-dioxane/L)ReferencePseudonocardia dioxanivorans CB11901.65 0.056.32 0.220.45 0.09487.14 173.45aBarajas-Rodrigu

15、ez et al.(2018)Mycobacterium dioxanotrophicus PH-06Not reported78.00 10.000.16Not reportedHe et al.(2018)Rhodococcus ruber 2195.00 0.240.015 0.030.24 0.020.49 1.16Simmer et al.(2021)Rhodococcus ruber 219s Monod kinetics make it a strong candidate for field-scale bioaugmentation37Can phytoremediation

16、+bioaugmentation with R.ruber 219 treat 1,4-dioxane-contaminated water at the pilot scale?38Can phytoremediation+bioaugmentation with R.ruber 219 treat 1,4-dioxane-contaminated water at the pilot scale?ESTCP Demonstration at former Twin Cities Army Ammunition Plant(TCAAP)Arden Hills,MNER21-509639Cre

17、dit:Ecolotree Inc.40Credit:Ecolotree Inc.41Credit:Ecolotree Inc.ESTCP Demonstration4243Credit:Ecolotree Inc.Each PhAGR planted with both hybrid poplar and willow75-gal HDPE Container filled with Perlite MixBioaugmentation with R.ruberScreened PVC monitoring well44Credit:Ecolotree Inc.45Credit:Ecolot

18、ree Inc.46Credit:Ecolotree Inc.4748495051525354Credit:Ecolotree Inc.55565758In-series phyto+bio system effectively treated influent dioxane for 9 weeks to as low 0.5 g/LSimmer et al.,Manuscript in progress5859qPCR analyses suggest sustained R.ruber population over 9 weeksSimmer et al.,Manuscript in

19、progress5960Bioaugmented phytoremediation effectively treated TCE to 0.2 g/LSimmer et al.,Manuscript in progress6061Bioaugmented phytoremediation effectively treated cDCE to 0.1 g/LSimmer et al.,Manuscript in progress6162Bioaugmented phytoremediation effectively treated 1,1,1-TCA 0.1 g/LSimmer et al

20、.,Manuscript in progress6263Minor vinyl chloride(1.0 g/L)formation observed in effluentSimmer et al.,Manuscript in progress63Major TakeawaysPhytoremediation system bioaugmented with R.ruber 219 capable of sustained remediation of dilute(50%)of dilute dioxane by R.ruber 219 for2 monthsTrees capable of complete transpiration(zero discharge),dioxane mass removalRoot zone may provide R.ruber with vitamin B1CVOCs effectively removed in all treatments,volatilization due to pumping likely played an important role83Credit:Ecolotree Inc.